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FRB-mCherry-FTH1

Plasmid

#100749

Purpose

Part of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tag

Depositor

Stephen Royle

Article

Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

Insert

ferritin heavy chain 1 (FTH1 Human)

Tags

FRB and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRF+423Dux4

Plasmid

#21625

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

Double homeobox, chr 4 (DUX4 Human)

Use

Luciferase

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pHR_PGK 3XFlag_4D5-5_CAR-mCherry

Plasmid

#247508

Purpose

Expresses a CAR against HER2 and mCherry

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

CAR against Her2 and mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-Pc-sfGFP

Plasmid

#199248

Purpose

Fluorescent labelling of bacterial cells with sfGFP

Depositor

Uwe Mamat

Article

Mamat et al Appl Environ Microbiol. 2023 May 17:e003

Insert

Codon-optimized sfGFP gene

Expression

Bacterial

Mutation

Codon optimization for Xanthomonadaceae

Promoter

Pc promoter from class III integron of Delftia a…

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ CSPG5 Neuroglycan 12960 VLVH synNotch

Plasmid

#247569

Purpose

Expresses a synNotch receptor binding to CSPG5 Neuroglycan

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch receptor binding to CSPG5 Neuoglycan

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA-FLAG-METTL3-APPA

Plasmid

#160251

Purpose

Express catalytic inactive FLAG-METTL3 in human cells

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

CMV

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHiUGE TurboID-HA donor (all-in-one) ORF0

Plasmid

#200383

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor (all-in-one) ORF2

Plasmid

#200388

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-Wild-Type

Plasmid

#235169

Purpose

Expresses Wild-Type GRP78 in mammalian cells

Depositor

Amy Shiu Lee

Article

Zhang et al J Biol Chem. 2010 May 14;285(20):15065-1

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

Plasmid

#184632

Purpose

Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gad67 (Gad1 Mouse)

Use

AAV

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV INS2x mRFP-3MREs (AAV-beta)

Plasmid

#206928

Purpose

Beta cell-specific gene expression from AAV in human pancreatic islets

Depositor

Markus Grompe

Article

Chai et al Hum Gene Ther. 2022 Aug;33(15-16):789-80

Insert

mRFP

Use

AAV

Expression

Mammalian

Promoter

INSx2

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-amyloid-β 40/42 nanobody (R3VQ) with sortase tag

Plasmid

#233217

Purpose

Mammalian epression of anti-amyloid-β 40/42 nanobody (R3VQ) with a sortase tag for direct dye conjugation.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv 2023.10.24.563674

Insert

Anti-amyloid-β 40/42 nanobody (R3VQ)

Tags

Sortase tag, His tag

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen

Plasmid

#231986

Purpose

Empty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreen

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

OVA-2A-ZsGreen

Use

Lentiviral

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHiUGE TurboID-HA donor (all-in-one) ORF1

Plasmid

#200384

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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HDAC4 Flag

Plasmid

#13821

Purpose

Mammalian expression of human histone deacetylase 4 with flag tag

Depositor

Eric Verdin

Article

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC4 (HDAC4 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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TRAPPC10_pcDNA6.2/EmGFP-Bsd

Plasmid

#176989

Purpose

Mammalian expression vector encoding TRAPPC10 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

TRAPPC10 (TRAPPC10 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_g5-HT3.0

Plasmid

#208726

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0

Use

AAV

Promoter

EF1a

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5B-HA-Smad2

Plasmid

#11734

Depositor

Jeff Wrana

Article

Eppert et al Cell. 1996 Aug 23. 86(4):543-52.

Insert

SMAD2 (SMAD2 Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 3, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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mKate2-DrCavin1b in pT3TS-Dest

Plasmid

#194295

Purpose

In vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAC

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1b (cavin1b Zebrafish)

Use

In vitro transcription of mrna

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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CD8-GFP-FKBP

Plasmid

#100751

Purpose

Expression of GFP-FKBP at the plasma membrane in mammalian cells. Plasma membrane targeting is via CD8 alpha chain.

Depositor

Stephen Royle

Article

Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

Insert

CD8A (CD8A Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Truncated

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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