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Plasmid#71665Purposeintronically embedded eSIBR backbone in gateway middle entry vector (pDONR221)DepositorInsertintronic eSIBR cassette
UseRNAi; Multi-site gatewayTagsExpressionMutationPromoterNoneAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMyc-CMV1
Plasmid#53582Purposemammalian expression vector with Myc tagDepositorTypeEmpty backboneUseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
ChrV_oxTi365_loxP_myo2_neoR
Plasmid#194057PurposeDual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genomeDepositorInsertoxTi365
UseTagsExpressionWormMutationPromoterNoneAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAH25-SceI
Plasmid#129389PurposeDerived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.DepositorInsertChloramphenicol acetyltransferase
UseTagsExpressionBacterialMutationN/APromoterCAT promoterAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSCL.140
Plasmid#184995PurposeExpress Cas9 in human cellsDepositorInsertSpCas9
UseTagsSV40NLSExpressionMammalianMutationPromoterTetOn-3gAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-AtUBQ10:GFP-T35S
Plasmid#211836PurposePlant transient expression cassette for GFP (control) under UBQ10 promoter.DepositorInsertGFP control
UseSynthetic BiologyTagsGFPExpressionPlantMutationPromoterUBIQUITIN10Available sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
CasRX_Pac
Plasmid#194803PurposeCasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.DepositorInsertCasRX
UseTagsExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYR14 (XII-5:TDH3p:his6-mKate2-LipPks1-IDP1t)
Plasmid#167357PurposeIntegrative plasmid for cassette TDH3p:his6-mKate2-LipPks1-IDP1tDepositorInsertTDH3p:his6-mKate2-LipPks1-IDP1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-eSIBR
Plasmid#71664PurposeEGFP coding region followed by enhanced synthetic inhibitory BIC/miR-155 RNA (eSIBR) backboneDepositorInsertEGFP coding sequence followed by eSIBR cassette
UseTagsExpressionMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pATF4-donor
Plasmid#113805PurposeCRISPR donor plasmid to tag human transcription factor ATF4 with GFPDepositorInsertATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
UseCRISPRTagsExpressionMutationrs140428747PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
LT0
Plasmid#210551PurposeBinary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporterDepositorInsertpU6::AAVS1gRNA::LT0::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT1
Plasmid#210552PurposeBinary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporterDepositorInsertpU6::AAVS1gRNA::LT1::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT2
Plasmid#210553PurposeBinary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporterDepositorInsertpU6::AAVS1gRNA::LT2::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonorTp
Plasmid#211788PurposepDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpRDepositorInsertdhfr
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYR11 (XII-5:TDH3p:his6-mKate2-MBP-IDP1t)
Plasmid#167356PurposeIntegrative plasmid for cassette TDH3p:his6-mKate2-MBP-IDP1tDepositorInsertTDH3p:his6-mKate2-MBP-IDP1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
BASIC_SEVA_66.11
Plasmid#194213PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOCC82
Plasmid#118862Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant proteinDepositorInsertNcoI-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSTK120-mCMV-TK
Plasmid#22054DepositorInsertmCMV-TK-pA expression cassette
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRE57I-GNAc
Plasmid#170637PurposeDNA donor for transpositionDepositorInsertMini-Tn6677, GNAc cassette, lacI
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pY3-LYS-IV
Plasmid#218119PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTC201B
Plasmid#52193Purposefor creating reversible targeting constructDepositorInsertpromoter-trap cassette Neo-Ires-GFP
UseCre/LoxTagsNeo, GFPExpressionMutationPromoterAvailable sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNCS Citrine
Plasmid#91761PurposeBacterial expression of a fluorescent protein, CitrineDepositorInsertCitrine
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdx1-mTQ2-P2A-FlpO-PA
Plasmid#67279PurposePancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon contextDepositorInsertsmTQ2=mturquoise2 blue fluorescent gene
FlpO recombinase
UseTagslinked to FlpO through an P2A ribosomal skipping …ExpressionMammalianMutationPromoterpdxAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDCC5
Plasmid#59984PurposeBi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassetteDepositorInsertCas9-D10A
UseCRISPRTagsHAExpressionInsectMutationD10APromoterhsp70BbAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
mUAVbla:tetR 5′
Plasmid#149379PurposemUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangsDepositorInserttetA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
EC2_7_dCas9_HC_sgRNA
Plasmid#163711PurposeHigh copy plasmid with dCas9 and sgRNA expression cassettesDepositorInsertdCas9
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-Blast-mU6
Plasmid#206806PurposepLentiCRISPRv2 variant with mU6 promoter driving the sgRNA, and a Blasticidin resistance cassetteDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICSL70008
Plasmid#50336DepositorInsertpFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICSL70004
Plasmid#50334DepositorInsertkanamycin/neomycin/paromomycin resistance cassette (Nos promoter (A. tumefaciens) + NptII (E.coli) + Ocs terminator (A. tumefaci
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJB01
Plasmid#111957PurposeExpresses His10-RFP-Halo; construct generated for pull-down and localization experimentsDepositorInsertRFP
UseTagsHaloTagExpressionBacterialMutationPromoterT7Available sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only