We narrowed to 9,753 results for: try

1. pYPQ233-STU

   Plasmid

   #138111

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated LbCas12a-SRDX repressor without promoter and terminator

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   dLbCas12a-SRDX
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-nEF-Coff/Fon DREADD Gi-mCherry

   Plasmid

   #177669

   Purpose

   Expresses DREADD hM4D (Gi) only in Flp expressing cells and not in Cre expressing cells

   Depositor

   Karl Deisseroth

   Article

   Knoedler et al Cell. 2022 Feb 17;185(4):654-671.e22. do

   Insert

   DREADD Gi-mCherry
   

   Use

   AAV, Cre/Lox, and Mouse Targeting ; Intrsect

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   nEF

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2xFlag CMV2-YAP2-1st&2nd WW mutant

   Plasmid

   #19048

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   Tryptophan 199 changed to Alanine,and Proline 202…

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC1-V16-eGFP

   Plasmid

   #122033

   Purpose

   AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and eGFP expression

   Depositor

   Liliane Tenenbaum

   Article

   Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

   Insert

   eGFP
   

   Use

   AAV

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcBac3-EcLeu TAG-EGFP**

   Plasmid

   #228785

   Purpose

   EcLeu aaRS/tRNA system for TAG suppression with EGFP containing two stop codons for dual suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcLeuRS
   EcLtR
   EGFP**
   

   Use

   Baculoviral

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Available Since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-neo:Beta2meGFP

   Plasmid

   #45096

   Depositor

   Henry Lester

   Article

   Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

   Insert

   nAChR beta2 (Chrnb2 Mouse)
   

   Expression

   Mammalian

   Mutation

   There are strain differences between C57BJ/6 and …

   Promoter

   CMV

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKK-TEV-EGFP

   Plasmid

   #105793

   Purpose

   Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-EGFP

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223 SARS-CoV-2 ORF3B

   Plasmid

   #141272

   Purpose

   Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF3B
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pT3EF5a-PIK3CA H1047R

   Plasmid

   #180034

   Purpose

   Entry vector of PIK3CA H1047R for gateway cloning.

   Depositor

   Xin Chen

   Article

   Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

   Insert

   PIK3CA H1047R (PIK3CA Human)
   

   Use

   Entry vector

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   H1047R

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. DYR1A_HUMAN_D0

    Plasmid

    #79690

    Purpose

    This plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    DYRK1A (DYRK1A Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pKK-FLAG-BirA-TEV

    Plasmid

    #105770

    Purpose

    Expression of your protein of interest in fusion with FLAG-BirA. FLAG-tagged at the N-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    FLAG-BirA-TEV

    Expression

    Mammalian

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC1-V16-Luc

    Plasmid

    #122034

    Purpose

    AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and Luciferase expression

    Depositor

    Liliane Tenenbaum

    Article

    Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

    Insert

    Luciferase
    

    Use

    AAV

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKK-TEV-ProteinA

    Plasmid

    #105788

    Purpose

    Expression of your protein of interest in fusion with two IgG binding domains of Staphylococcus aureus protein A at the C-terminus (cleavable by TEV) (PMID: 3507693, 10504710). Protein purification.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-ProteinA

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKK-mCherry-TEV

    Plasmid

    #105779

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mCherry-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKK-BI16-TEV-mCherry

    Plasmid

    #105800

    Purpose

    Concomitant expression of two proteins. One protein is expressed with mCherry fusion tag (at the C-terminus), cleavable by TEV protease; second tag introduced by user.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCherry

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223 SARS-CoV-2 S_nostop

    Plasmid

    #152988

    Purpose

    Gateway-compatible Entry vector, with insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. AURKA_HUMAN_D0

    Plasmid

    #79739

    Purpose

    This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    AURKA (AURKA Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pLV_iRFP_P2AT2A_mScarlet-I_Myl9

    Plasmid

    #172474

    Purpose

    Mammalian expression of myosin light chain (Myl9) tagged with mScarlet-I and cytoplasmic iRFP as a reference marker.

    Depositor

    Sean R Collins

    Article

    Hadjitheodorou et al Nat Commun. 2021 Nov 16;12(1):6619. doi:

    Inserts

    iRFP670
    mScarlet-I-Myl9 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    EF1alpha (with UCOE) and none (same transcript as…

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEN_TT 3XFLAG-wtGATA6-3XAU1

    Plasmid

    #72612

    Purpose

    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3XAU1 and 3XFLAG

    Expression

    Bacterial

    Promoter

    TRE tight promoter

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. BcLOV4 fusion tools screening plasmid #2

    Plasmid

    #174512

    Purpose

    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
    

    Tags

    3xFLAG and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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