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pEGFP-C1-FLAG-GFP-Ubl4A-N(1-89)

Plasmid

#86922

Purpose

To express Ubl4A (1-89 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 90-157 is deleted

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pSMART-ala11

Plasmid

#87172

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proA

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSMART-ala12

Plasmid

#87173

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proC

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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const #7-RFP

Plasmid

#81081

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23109

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #6-RFP

Plasmid

#81080

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23117

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pWHI2-HYG

Plasmid

#80589

Purpose

CEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoter

Depositor

John McCusker

Article

Vijayraghavan et al Genetics. 2018 Nov 29. pii: genetics.118

Insert

WHI2 (WHI2 Budding Yeast)

Expression

Yeast

Promoter

WHI2 native promoter

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
pET24_BD3179

Plasmid

#73142

Purpose

Expresses in E.coli B. bacteriovorus Bd3179

Depositor

Sandra Gabelli

Article

de la Peña et al PLoS One. 2015 Nov 2;10(11):e0141716. do

Insert

Bd3179

Expression

Bacterial

Available Since

March 22, 2016

Availability

Academic Institutions and Nonprofits only
pRFP shRNA-resistant TrkB F616A -S478A

Plasmid

#72718

Purpose

Mammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant and lacking TIAM1-binding site

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

TrkB

Tags

RFP

Expression

Mammalian

Mutation

shRNA-resistant TrkB (T300C, T301A, C302G, C306A,…

Promoter

CMV

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
FCIV1-GR SS287AA

Plasmid

#72703

Purpose

Lentiviral expression of GR Phospho-deficient mutant (2nd generation)

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Mutation

SS287AA

Promoter

ubiquitin

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
MS4A1 RFP (805)

Plasmid

#62009

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

Insert

MS4A1

Tags

mRFP

Expression

Mammalian

Promoter

pgk

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pET30-SBL1(160E,HisTagDel)

Plasmid

#66879

Purpose

Expresses a version of pET30Xa-SBL1(160E) with 41 bp Histag removed

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160E)

Expression

Bacterial

Mutation

S160E

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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ptCMV-136/63-VR-HD

Plasmid

#50699

Purpose

Final destination vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Use

TALEN

Tags

Flag

Promoter

CMV

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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ptCMV-136/63-VR-NG

Plasmid

#50700

Purpose

Final destination vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Use

TALEN

Tags

Flag

Promoter

CMV

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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ptCMV-136/63-VR-NI

Plasmid

#50701

Purpose

Final destination vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Use

TALEN

Tags

Flag

Promoter

CMV

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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ptCMV-136/63-VR-NN

Plasmid

#50702

Purpose

Final destination vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Use

TALEN

Tags

Flag

Promoter

CMV

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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ptCMV-153/47-VR-HD

Plasmid

#50703

Purpose

Final destination vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Use

TALEN

Tags

Flag

Promoter

CMV

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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ptCMV-153/47-VR-NI

Plasmid

#50705

Purpose

Final destination vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Use

TALEN

Tags

Flag

Promoter

CMV

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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ptCMV-153/47-VR-NN

Plasmid

#50706

Purpose

Final destination vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Use

TALEN

Tags

Flag

Promoter

CMV

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR4_EWSR1_C2G_R650G

Plasmid

#26371

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

EWSR1 (EWSR1 Human)

Use

Entry vector

Mutation

R650G

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pJC25R1+4stop

Plasmid

#17723

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+4 (SUP35 Budding Yeast)

Expression

Bacterial

Mutation

E.coli expression plasmid for NM-region of Sup35 …

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pLN428 (FuGW-G5p-mKate2-1Pe )

Plasmid

#105184

Purpose

Module 3 - GAL4 synthetic promoter (with five binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G5p-mKate2-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pLN434 (FuGW-G14p-mKate2-1Pe )

Plasmid

#105188

Purpose

Module 3 - GAL4 synthetic promoter (with fourteen binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G14p-mKate2-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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Golden Gate TALEN and TAL effector kit 2.0

Plasmid Kit

#1000000024

Purpose

Used to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats. Uses Golden Gate cloning method and has been validated in multiple organisms.

Depositor

Adam Bogdanove, Daniel Voytas

Available Since

Jan. 8, 2013

Availability

Academic Institutions and Nonprofits only
pSpCas9(BB)-2A-Puro (PX459) V2.0

Plasmid

#62988

Purpose

Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Has Service

Cloning Grade DNA

Insert

hSpCas9-2A-Puro V2.0

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-iCas9-neo

Plasmid

#85400

Purpose

Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence

Depositor

Qin Yan

Article

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Insert

Flag-iCas9-P2A-GFP

Use

Lentiviral

Tags

Flag-iCas9-P2A-GFP

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCXWB-EBNA1

Plasmid

#37624

Purpose

Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

EBNA-1 (EBNA-1 human herpesvirus 4)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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HA-Ubiquitin

Plasmid

#18712

Purpose

Mammalian expression of human ubiquitin with HA tag

Depositor

Edward Yeh

Article

Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-6

Insert

ubiquitin (UBB Human)

Tags

HA

Expression

Mammalian

Mutation

The HA-Ubiquitin insert is from the UBB gene. The…

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0

Plasmid

#62987

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER Short P2A

Plasmid

#164108

Purpose

Detect the short Mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Mt-ER Short P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER Long P2A

Plasmid

#164107

Purpose

Detect the long Mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Mt-ER Long P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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