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We narrowed to 7,002 results for: tac

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Showing: 3961 - 3980 of 7002 results

  11. pLD-puro-Cc-CR-PARK7WT-VA

    Plasmid
    #115187
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7WT into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7WT (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115187-plasmid-map-sequence-id-225414

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  12. pLD-puro-Cc-CR-PARK7V51G-VA

    Plasmid
    #115188
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7V51G into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7V51G (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115188-plasmid-map-sequence-id-225424

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  13. pLD-puro-Cc-CR-PARK7C53A-VA

    Plasmid
    #115189
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7C53A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7C53A (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115189-plasmid-map-sequence-id-225429

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  14. pLD-puro-Cc-CR-PARK7H126A-VA

    Plasmid
    #115190
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7H126A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7H126A (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115190-plasmid-map-sequence-id-225431

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  15. pLD-puro-Cc-CR-PARK7E163K-VA

    Plasmid
    #115191
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7E163K into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7E163K (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115191-plasmid-map-sequence-id-225432

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  16. hNTa1-qgRNA-pYJA5

    Plasmid
    #217779
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217779-plasmid-map-sequence-id-466021

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  18. pET28 E2 LBD-Tev-E1a-His

    Plasmid
    #232117
    Purpose
    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    DBT (DBT Human)
    Tags
    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232117-plasmid-map-sequence-id-465790

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  19. pαH-S-RRAR-OMICRON.BA.5

    Plasmid
    #213071
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 with with furin side intact
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    pαH-S-RRAR-OMICRON.BA.5 (S )
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685, furin site inta…
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    213071-plasmid-map-sequence-id-422361

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  20. pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin

    Plasmid
    #237480
    Purpose
    Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)
    Use
    Lentiviral
    Tags
    IRES-mNeonGreen-NES/PKI and P2A-Blasticidin
    Expression
    Mammalian
    Mutation
    S484D + S813I + deleted amino acids 68-76 + 676-6…
    Promoter
    CMV
    Available Since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    237480-plasmid-map-sequence-id-477747

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Showing: 3961 - 3980 of 7002 results