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We narrowed to 173 results for: pef gfp

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Showing: 51 - 100 of 173 results

  1. pEf1a-superDn29-dCas9-P2A-GFP

    Plasmid
    #247156
    Purpose
    Mammalian expression of a human codon optimized engineered superDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Publication
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    superDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…
    Promoter
    Ef1a
    Available since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247156-plasmid-map-sequence-id-491689

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  2. pEf1a-hifiDn29-dCas9-P2A-GFP

    Plasmid
    #247158
    Purpose
    Mammalian expression of a human codon optimized engineered hifiDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Publication
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hifiDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…
    Promoter
    Ef1a
    Available since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247158-plasmid-map-sequence-id-491688

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  6. pEF1-Pls4-sfGFP

    Plasmid
    #205751
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls4 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. High strength.
    Depositor
    Jerome Bonnet
    Publication
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls4 (Phyperspank mutant, IPTG inducible)
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    205751-plasmid-map-sequence-id-478437

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  7. pEF-GalNAcT2-msGFP2

    Plasmid
    #218965
    Purpose
    Expresses full length GalNAcT2-msGFP2 mammalian cells
    Depositor
    Benjamin Glick
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GalNAcT2-msGFP2 (GALNT2 Human)
    Tags
    msGFP2
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218965-plasmid-map-sequence-id-446216

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  8. pEF-GalNAcT2-moxGFP2

    Plasmid
    #218966
    Purpose
    Expresses full length GalNAcT2-moxGFP2 mammalian cells
    Depositor
    Benjamin Glick
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GalNAcT2-moxGFP2 (GALNT2 Human)
    Tags
    moxGFP2
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218966-plasmid-map-sequence-id-445673

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  9. pEf1a-goldDn29-dCas9-P2A-GFP

    Plasmid
    #247157
    Purpose
    Mammalian expression of a human codon optimized engineered goldDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Publication
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    goldDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N
    Promoter
    Ef1a
    Available since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247157-plasmid-map-sequence-id-491683

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  18. pLenti-EGFP-2xFYVE

    Plasmid
    #136996
    Purpose
    Lentiviral EGFP lipid sensor for PI(3)P
    Depositor
    Ken-Ichi Takemaru
    Insert
    EGFP-2xFYVE
    Use
    Lentiviral
    Tags
    EGFP
    Promoter
    EF1 alpha
    Available since
    Feb. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    136996-plasmid-map-sequence-id-270659

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  23. pEF1-Pls2-sfGFP

    Plasmid
    #205749
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls2 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/low strength.
    Depositor
    Jerome Bonnet
    Publication
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls2 (Phyperspank mutant, IPTG inducible)
    Available since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    205749-plasmid-map-sequence-id-483890

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  27. pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB

    Plasmid
    #169921
    Purpose
    DNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.
    Depositor
    Zhili Rong
    Publication
    Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IRES-Puro-pEF1a-EGFP
    Use
    CRISPR
    Promoter
    EF1a
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    169921-plasmid-map-sequence-id-336026

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  38. pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA

    Plasmid
    #176461
    Purpose
    Vector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMV
    Depositor
    Hui Yang
    Publication
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Insert
    BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176461-plasmid-map-sequence-id-346306

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  40. C. elegans miniMos transgenesis kit

    Plasmid kit
    #1000000031
    Purpose
    Transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon.
    Depositor
    Erik Jorgensen
    Application
    Genome Editing
    Vector type
    Worm Expression
    Editing type
    Transposon
    Available since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only

  41. pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid
    #176459
    Purpose
    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMV
    Depositor
    Hui Yang
    Publication
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176459-plasmid-map-sequence-id-346304

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  42. pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid
    #176460
    Purpose
    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMV
    Depositor
    Hui Yang
    Publication
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176460-plasmid-map-sequence-id-346305

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  46. pEF-XMAS-2xStop

    Plasmid
    #164412
    Purpose
    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.
    Depositor
    Xiao Wang
    Publication
    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology
    Tags
    T2A-GFP
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    164412-plasmid-map-sequence-id-338860

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  48. pEF1α-ancestral-retroCHMP3-HA

    Plasmid
    #154248
    Purpose
    expresses ancestral New World monkey retroCHMP3 in mammalian cells
    Depositor
    Wesley Sundquist
    Publication
    Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    reconstructed ancestral sequence of New World monkey retroCHMP3
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    154248-plasmid-map-sequence-id-328994

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Showing: 51 - 100 of 173 results