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Plasmid#99508PurposeBacterial expression of hydroxylated Hif1a TAD (776-826)DepositorUseTagsHis6GB1ExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only
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p(HA)HIF1alpha(401delta603) (L795V, C800S, L818S, L822V)
Plasmid#52216Purposemammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and LCLL mutationDepositorInsertHIF1alpha (401delta603) LCLL (HIF1A Human)
UseTagsHAExpressionMammalianMutationaa 401-603 deleted, removes the oxygen-dependent …PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-Hif1a(776-826) + CBP TAZ1(340-439)
Plasmid#99343PurposeBacterial expression of mouse CBP TAZ1 domain as coexpression with Hif1a TADDepositorInsertsUseTagsHis6GB1ExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-Fusion + CBP TAZ1(340-439)
Plasmid#173762PurposeBacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructsDepositorUseTagsHis6GB1 and thrombinExpressionBacterialMutationfusion of CITED2 and Hif1aPromoterT7Available sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-FusionL21A + CBP TAZ1(340-439)
Plasmid#173763PurposeBacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructsDepositorUseTagsHis6GB1 and thrombinExpressionBacterialMutationfusion of CITED2 and Hif1aPromoterT7Available sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
HIsGB1-FusionL63A + CBP TAZ1(340-439)
Plasmid#173764PurposeBacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructsDepositorUseTagsHis6GB1 and thrombinExpressionBacterialMutationfusion of CITED2 and Hif1aPromoterT7Available sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-RO1.7-dnHIF
Plasmid#206343PurposeAAV plasmid expressing dominant negative HIF1A in photoreceptorsDepositorInsertdominant negative hif1a
UseAAVTagsExpressionMutationdominant negative HIF1APromoter1.7kb human red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
ODD-Luciferase-pcDNA3
Plasmid#18965DepositorInsertODD-Firefly Luciferase (HIF1A Human)
UseTagsExpressionMammalianMutationHIF1a ODD sequence fused in frame to luciferase O…PromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE9
Plasmid#90343PurposeHIF1a - Hypoxia gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterHIF1aAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HIF1 alpha
Plasmid#21101DepositorInsertHYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HIF1 alpha K532R
Plasmid#21102DepositorInsertHYPOXIA INDUCIBLE FACTOR 1 ALPHA-K532R MUTANT (HIF1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
TFORF3297
Plasmid#144773PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHIF1A (HIF1A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0481
Plasmid#142638PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHIF1A (HIF1A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0480
Plasmid#143705PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHIF1A (HIF1A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3296
Plasmid#144772PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHIF1A (HIF1A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
HIF-1alpha-Clover
Plasmid#87215PurposeC-terminally Clover-tagged human HIF-1aDepositorInsertHIF-1a (HIF1A Human)
UseTagsCloverExpressionMammalianMutationL40F, M88V, and Q814RPromoterCMVAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pg-nucODD-EGFP
Plasmid#87212PurposeHIF-1a sequence without the bHLH domain fused to EGFPDepositorInsertHIF-1a nucODD (HIF1A Human)
UseTagsEGFPExpressionMammalianMutationwithout the bHLH domain, Q814RPromoterCMVAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-1alpha Wildtype
Plasmid#163365PurposeExpress HA- and Clover-tagged HIF-1alpha wildtype plasmidDepositorInsertHypoxia Inducible Factor 1 alpha (HIF1A Human)
UseTagsHA- and Clover-taggedExpressionMammalianMutationWildtypePromoterCMV PromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
HRE-oUnaG
Plasmid#201712PurposeHypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1aDepositorInsertUnaG
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-1alpha S31D
Plasmid#163370PurposeExpress HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser31Asp) plasmidDepositorInsertHypoxia Inducible Factor 1 alpha S31D (HIF1A Human)
UseTagsHA- and Clover-taggedExpressionMammalianMutationS31DPromoterCMV PromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only