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Showing: 21 - 40 of 2386 results

  3. EKARdual

    Plasmid
    #99870
    Purpose
    Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate
    Depositor
    Marc Tramier
    Article
    Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10
    Insert
    EKAR
    Use
    Tags
    ShadowG and mTFP1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    99870-plasmid-map-sequence-id-182629

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  4. AKARdual

    Plasmid
    #99869
    Purpose
    Measure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrate
    Depositor
    Marc Tramier
    Article
    Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10
    Insert
    AKAR4
    Use
    Tags
    LSSmOrange and mKate2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    99869-plasmid-map-sequence-id-192827

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  5. pTO149_RFP_CauNEO

    Plasmid
    #177277
    Purpose
    Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.
    Depositor
    Teresa O'Meara
    Article
    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:
    Insert
    RFP, CauNEO
    Use
    Tags
    ENO1-RFP
    Expression
    Bacterial
    Mutation
    All CUG Leucine codons were replaced with the syn…
    Promoter
    AgTEF2
    Available since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    177277-plasmid-map-sequence-id-347241

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  6. pRDA_221

    Plasmid
    #169142
    Purpose
    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.
    Depositor
    John Doench, David Root
    Insert
    EGFP , EGFP sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    169142-plasmid-map-sequence-id-334957

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  9. pDBD-M

    Plasmid
    #50572
    Purpose
    Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571
    Depositor
    Alexander Varshavsky
    Article
    Turner et al Science. 2000 Sep 22;289(5487):2117-20.
    Insert
    DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Gal1
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pDBD-R

    Plasmid
    #50571
    Purpose
    Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572
    Depositor
    Alexander Varshavsky
    Article
    Turner et al Science. 2000 Sep 22;289(5487):2117-20.
    Insert
    DHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
    Use
    Tags
    Expression
    Yeast
    Mutation
    M1R (B-gal)
    Promoter
    Gal1
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pCL27-decoder

    Plasmid
    #159449
    Purpose
    Insert: Pfus1tet-citrine-tCyc1. The fluorescent protein Citrine is inducible by alpha-factor and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    Pfus1tet-citrine-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pfus1tet
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159449-plasmid-map-sequence-id-313197

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Showing: 21 - 40 of 2386 results