We narrowed to 582 results for: Pef1

1. pEF1a-mRor2Y641F

   Plasmid

   #22635

   Depositor

   Roel Nusse

   Article

   Mikels et al J Biol Chem. 2009 Aug 31. ():.

   Insert

   Ror2 Y641F (Ror2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed Tyrosine 641 to Phenylalanine

   Promoter

   Available since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pEF1a-mRor2Y645F

   Plasmid

   #22636

   Depositor

   Roel Nusse

   Article

   Mikels et al J Biol Chem. 2009 Aug 31. ():.

   Insert

   Ror2 Y645F (Ror2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed Tyrosine 645 to Phenylalanine

   Promoter

   Available since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pEF1a-mRor2Y646F

   Plasmid

   #22637

   Depositor

   Roel Nusse

   Article

   Mikels et al J Biol Chem. 2009 Aug 31. ():.

   Insert

   Ror2 Y646F (Ror2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed Tyrosine 646 to Phenylalanine

   Promoter

   Available since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pEF1a-IRES-Neo

   Plasmid

   #28019

   Depositor

   Thomas Zwaka

   Article

   Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF1a-BirA-V5-neo

   Plasmid

   #100548

   Purpose

   Expresses C-terminal V5-tagged BirA biotin ligase

   Depositor

   Jian Xu

   Article

   Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

   Insert

   C-terminal V5-tagged BirA
   

   Use

   Tags

   V5, His

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1a-FB-dCas9-puro

   Plasmid

   #100547

   Purpose

   Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein

   Depositor

   Jian Xu

   Article

   Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

   Inserts

   N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
   puromycin
   

   Use

   CRISPR

   Tags

   FLAG, Biotin-acceptor-site (Avitag)

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEF1-alpha-IIb

   Plasmid

   #27288

   Depositor

   Timothy Springer

   Article

   Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

   Insert

   integrin alpha IIb (ITGA2B Human)
   

   Use

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEF1a-Usp9x_CD-mCherry

   Plasmid

   #170380

   Purpose

   Vector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.

   Depositor

   Miguel Ramalho-Santos

   Article

   Macrae et al Nat Commun. 2021 Mar 25;12(1):1865. doi:

   Insert

   Ubiquitin specific protease 9x catalytic domain (Usp9x Mouse)
   

   Use

   Tags

   mCherry-3xFlag

   Expression

   Mammalian

   Mutation

   Catalytic domain

   Promoter

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEF1a_FL MUT TET1

   Plasmid

   #124081

   Purpose

   Expression of catalytic inactive form of full-length TET1

   Depositor

   Silvia Monticelli

   Article

   Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

   Insert

   TET1 - full length, mutated (TET1 Human)
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   H1672Y, D1674A

   Promoter

   EF1 alpha

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF1a_CD MUT TET1

    Plasmid

    #124083

    Purpose

    Expression of mutated catalytic domain only form of TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 - catalytic domain mutated (TET1 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    H1672Y, D1674A

    Promoter

    EF1 alpha

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEF1a-2xSV40_NLS-NLuc

    Plasmid

    #135953

    Purpose

    Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Use

    Tags

    2xSV40-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEF1-His-NEDD8

    Plasmid

    #159322

    Purpose

    Mammalian cell expression of mouse His-NEDD8

    Depositor

    Jianjun Zhao

    Insert

    His-NEDD8 (Nedd8 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEF1α-mouse-retroCHMP3-HA

    Plasmid

    #154178

    Purpose

    expresses mouse retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEF1a-2xSV40_NLS-CeNLuc

    Plasmid

    #135952

    Purpose

    Expresses the CeNLuc LumiFluor targeted to the nucleus in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    CeNLuc LumiFluor
    

    Use

    Tags

    2xSV40-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEF1-MH.Bl-mDcrSOM

    Plasmid

    #120540

    Purpose

    protein expression

    Depositor

    Petr Svoboda

    Insert

    Dicer (Dicer1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEF1-MH.Bl-mDcrOO

    Plasmid

    #120541

    Purpose

    protein expression

    Depositor

    Petr Svoboda

    Insert

    Dicer (Dicer1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEF1α-ancestral-retroCHMP3-HA

    Plasmid

    #154248

    Purpose

    expresses ancestral New World monkey retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

    Insert

    reconstructed ancestral sequence of New World monkey retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEF1-puro/hpb1

    Plasmid

    #59976

    Purpose

    integrin b1 short headpiece expression (beta-I, hybrid, PSI and I-EFG1 domains) expression

    Depositor

    Timothy Springer

    Insert

    human integrin b1 headpiece (ITGB1 Human)
    

    Use

    Tags

    Base coiled-coil and His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a promoter

    Available since

    Sept. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pEF1a-CRY2PHR-SID4X

    Plasmid

    #63663

    Purpose

    AAV expression of CRYS2PHR-SID4X

    Depositor

    Dong Kong

    Article

    Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

    Insert

    SID4X-CYR2PHR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF1α -NWM-Node 1

    Plasmid

    #154253

    Purpose

    expresses reconstructed New World monkey retroCHMP3 Node 1 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

    Insert

    reconstructed Node 1 of New World monkey retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge