We narrowed to 22,774 results for: Sis

1. pFS_0350_pET30-RT-CRISPR_Cellulomonas_bogoriensis_69B4_ARRAY_1_RC

   Plasmid

   #116962

   Purpose

   Expresses CbRT-Cas1-Cas2 under pT7lac promoter, encodes CbCRISPR array 1 RC, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Cellulomonas bogoriensis 69B4 RT-Cas1-Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry_C1 I3(resist)

   Plasmid

   #61399

   Purpose

   Expresses mCherry tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

   Insert

   Inhibitor-3 (PPP1R11 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   silent mutations of bases C183T T192C T189C

   Promoter

   CMV

   Available Since

   Sept. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

3. DHFR Saturation Mutagenesis Library - WT TYMS

   Pooled Library

   #1000000194

   Depositor

   Kimberly Reynolds

   Available Since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

4. pEXSISERS_mGreenLantern_FRT-PuroR-HSV-TK

   Plasmid

   #177868

   Purpose

   Cloning Backbone for mGreenLantern-based EXSISERS containing FRT-sites flanked PuroR-HSV-TK cassette; clone homology arms via BbsI (or BpiI).

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

   Insert

   mGreenLantern-based EXSISERS
   

   Use

   CRISPR, Synthetic Biology, and Unspecified

   Tags

   OLLAS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-SisTnpB1–ωRNA

   Plasmid

   #228365

   Purpose

   Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

   Depositor

   Tao Liu

   Article

   Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

   Insert

   SisTnpB1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRG705_lexO-AsiSI_LexA-TF_LEU2MX

   Plasmid

   #154817

   Purpose

   Inducible expression of AsiSI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AsiSI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. hHSF1 RNAi resistant

   Plasmid

   #118347

   Purpose

   This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).

   Depositor

   Lea Sistonen

   Article

   Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   RNAi resistance mutations (does not change amino …

   Promoter

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. BAP1-wt (siRNA resistant)

   Plasmid

   #108439

   Purpose

   Stable expression of BAP1-wt in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

   Insert

   BAP1 (BAP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c.1641C>T, c.1644T>A, c.1647T>C, c.1650C…

   Promoter

   CMV

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.2-Cx43-siResist

   Plasmid

   #49852

   Purpose

   Encodes human connexin 43 with wobble mutations conferring resistance against an siRNA

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   Connexin 43 (GJA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains several wobble mutations to confer resis…

   Promoter

   CMV

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pRS414 GPD-Sis1

    Plasmid

    #18687

    Depositor

    Susan Lindquist

    Article

    Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

    Insert

    Sis1 (SIS1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pDONR223 SCAF8_isoC_CRISPR resistant

    Plasmid

    #122466

    Purpose

    pDONR for Gateway cloning

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF8 isoform C CRISPR resistant (SCAF8 Human)
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Synonymous mutations conferring resistance to gRN…

    Promoter

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. sg resistant gamma-tubulin

    Plasmid

    #104433

    Purpose

    For expressing gamma-tubulin in cells expressing γTUBULIN single guide RNA

    Depositor

    Maria Alvarado-Kristensson

    Article

    Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    changed t bp28 to c; g bp30 to a; c bp33 to a; …

    Promoter

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS414 GPD-Sis1deltaG/F

    Plasmid

    #18688

    Depositor

    Susan Lindquist

    Article

    Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

    Insert

    Sis1 deltaG/F
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Sis1 with the G/F region deleted

    Promoter

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

14. MPS1 Candia parapsilosis

    Plasmid

    #232153

    Purpose

    MPS1 from Candia parapsilosis codon optimized for S. cerevisiae in pUCIDT

    Depositor

    Mark Winey

    Article

    Fabritius et al PLoS One. 2024 Mar 26;19(3):e0301084. do

    Insert

    MPS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHIV(T9)-DERA-sgresis

    Plasmid

    #222622

    Purpose

    Lentiviral vector that expresses sgRNA resistant DERA in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

    Insert

    DERA (DERA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. Lenti-sgSis#2/Cre

    Plasmid

    #193238

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Sis gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgSis#2 (Sis Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-sgSis#1/Cre

    Plasmid

    #193237

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Sis gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgSis#1 (Sis Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-rUSP19ER-siRNAresistant

    Plasmid

    #155251

    Purpose

    for mammalian overexpression of the siRNA resistant ER isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (ER isoform) siRNA resistant (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …

    Promoter

    CMV

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-rUSP19CYT-siRNAresistant

    Plasmid

    #155252

    Purpose

    for mammalian overexpression of the siRNA resistant CYT isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (CYT isoform) siRNA resistant (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …

    Promoter

    CMV

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223 SCAF4_CRISPR resistant

    Plasmid

    #122465

    Purpose

    pDONR for Gateway cloning

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF4 CRISPR resistant (SCAF4 Human)
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Synonymous mutations conferring resistance to gRN…

    Promoter

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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