We narrowed to 496 results for: blasticidin resistance

1. pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

   Plasmid

   #187949

   Purpose

   SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   SMASh-SpdCas9-tagRFPt-P2A-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSIR-bsr

   Plasmid

   #176507

   Purpose

   Self-inactivating retrovirus vector containing a Blasticidin S-resistance gene

   Depositor

   Hodaka Fujii

   Article

   Fujii et al Biol Methods Protoc. 2021 Dec 6;6(1):bpa

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCas9-Blast

   Plasmid

   #52962

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Lentiviral Prep

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

   Plasmid

   #187960

   Purpose

   AID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

   Plasmid

   #187963

   Purpose

   mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Inserts

   AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
   osTIR1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker and T2A

   Expression

   Mammalian

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCas9n(D10A)-Blast

   Plasmid

   #63593

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, nickase mutant

   Promoter

   EFS-NS

   Available Since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. B1979

   Plasmid

   #59181

   Purpose

   Expresses optimized blasticidin S resistance gene (BSD) for C. elegans

   Depositor

   Craig Mello

   Article

   Kim et al Genetics. 2014 May 30. pii: genetics.114

   Insert

   Blasticidin S resistance
   

   Expression

   Worm

   Promoter

   rpl-28

   Available Since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. FFiBlast p5

   Plasmid

   #68470

   Purpose

   Inverted and floxed blasticidin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Blasticidin Resistance
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Mutation

   Inverted

   Promoter

   LTR

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCWX-R4DESTR2-PGK-BSD

   Plasmid

   #114320

   Purpose

   Lentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Blasticidin resistance

   Depositor

   Patrick Salmon

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVU/myc

    Plasmid

    #24179

    Depositor

    Lars Ittner

    Insert

    Chloramphenicol resistance gene (CmR) - ccdB gene
    

    Use

    Lentiviral; Gateway

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pBS SK mCherryROSAbsr

    Plasmid

    #54321

    Purpose

    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.

    Depositor

    Silvestro Conticello

    Article

    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

    Insert

    mCherry and BlasticidinS-resistance
    

    Use

    CRISPR, Mouse Targeting, and TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. FFiBlast MSCV Puro

    Plasmid

    #68464

    Purpose

    Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Blasticidin Resistance
    

    Use

    Cre/Lox and Retroviral

    Expression

    Mammalian

    Mutation

    Inverted

    Promoter

    LTR

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pINS-Blast

    Plasmid

    #31388

    Depositor

    Yegor Vassetzky

    Article

    Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

    Insert

    bsd
    

    Use

    Cre/Lox

    Available Since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pML646

    Plasmid

    #206999

    Purpose

    Contains mAID-tag, mouse Ptbp1-specific homology arms and blasticidin S resistance gene.

    Depositor

    Eugene Makeyev

    Article

    Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

    Insert

    BSD resistance
    

    Use

    Unspecified

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBSVSG221KOblast

    Plasmid

    #59733

    Purpose

    For replacement of VSG221 with a blasticidin resistence gene in the VSG221 expression site in T.brucei.

    Depositor

    Gloria Rudenko

    Insert

    VSG221 knockout construct
    

    Use

    For homologous recombination into vsg221 expressi…

    Available Since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPTbsr

    Plasmid

    #117696

    Purpose

    Blasticidin-S resistance Vector for Phaeodactylum tricornutum

    Depositor

    Peter Kroth

    Article

    Buck et al PeerJ. 2018 Nov 14;6:e5884. doi: 10.7717

    Type

    Empty backbone

    Use

    Vector for phaeodactylum tricornutum

    Promoter

    fcpB

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221_SMAD2_HAe2_Bsd

    Plasmid

    #167166

    Purpose

    Blasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.

    Depositor

    Alexander Loewer

    Insert

    Repair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
    

    Use

    Repair template for gene editing in human cells

    Tags

    blasticidin

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-R2L3-IRES-BSD-pA

    Plasmid

    #139534

    Purpose

    Provides IRES-BSD-pA cassette on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    Blasticidin resistant gene
    

    Expression

    Mammalian

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid

    #106477

    Purpose

    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCerulean3-Bik-s2193

    Plasmid

    #177425

    Purpose

    To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bik, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Tags

    mCerulean3

    Expression

    Mammalian

    Promoter

    Human ferritin

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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