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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,840 results for: donor

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Showing: 21 - 40 of 4840 results

  6. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

    Plasmid
    #160736
    Purpose
    Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Publication
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    160736-plasmid-map-sequence-id-326992

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  9. pTC217

    Plasmid
    #70018
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Expression
    Plant
    Available since
    March 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    70018-plasmid-map-sequence-id-124557

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  12. pTC223

    Plasmid
    #70019
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Expression
    Plant
    Available since
    Dec. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    70019-plasmid-map-sequence-id-124558

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  15. pORANGE Tubb3-GFP KI

    Plasmid
    #131497
    Purpose
    Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)
    Depositor
    Harold MacGillavry
    Publication
    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
    Expression
    Mammalian
    Promoter
    U6 and Cbh
    Available since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    131497-plasmid-map-sequence-id-257260

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Showing: 21 - 40 of 4840 results