We narrowed to 54,048 results for: plasmid

1. ATP1A1_plasmid_donor_RD

   Plasmid

   #86551

   Purpose

   Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 (ATP1A1 Human)
   

   Use

   CRISPR and TALEN; Co-selection via hdr using ouab…

   Tags

   Expression

   Mammalian

   Mutation

   Q118R, N129D

   Promoter

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. ptetmCherry_prey_plasmid-2

   Plasmid

   #34617

   Depositor

   Lingchong You

   Article

   Song et al Nat Chem Biol. 2009 Dec;5(12):929-35. Ep

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ptet

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Receiver_plasmid1

   Plasmid

   #140694

   Purpose

   Encodes constitutive red (mCherry) fluorescence for identification

   Depositor

   Matthew Bennett

   Article

   Alnahhas et al ACS Synth Biol. 2019 Sep 20;8(9):2051-20

   Insert

   mCherry
   

   Use

   Tags

   ssrA degradation tag (ASV)

   Expression

   Bacterial

   Mutation

   Promoter

   pIq constitutive promoter

   Available since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Sender_plasmid1

   Plasmid

   #140692

   Purpose

   Encodes constitutive yellow (sfYFP) fluorescence for identification

   Depositor

   Matthew Bennett

   Article

   Alnahhas et al ACS Synth Biol. 2019 Sep 20;8(9):2051-20

   Insert

   sfYFP
   

   Use

   Tags

   ssrA degradation tag (ASV)

   Expression

   Bacterial

   Mutation

   Promoter

   pIq constitutive promoter

   Available since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Accessory plasmid 1

   Plasmid

   #128569

   Purpose

   for mixing with SPECS library to produce lentivirus

   Depositor

   Timothy Lu

   Article

   Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

   Insert

   ECFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Equalizer-L plasmid

   Plasmid

   #169732

   Purpose

   Equalizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Equalizer-H plasmid

   Plasmid

   #169367

   Purpose

   Equalizer variant plasmid with high eGFP output levels but reduced gene dosage compensation capability.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Equalizer-M plasmid

   Plasmid

   #169731

   Purpose

   Equalizer variant plasmid with medium eGFP output levels and medium gene dosage compensation capability.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Accessory plasmid 2

   Plasmid

   #128571

   Purpose

   for cloning identified individual SPECS

   Depositor

   Timothy Lu

   Article

   Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagRFP

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB4k5_RNAnano (r-plasmid)

    Plasmid

    #127634

    Purpose

    Encodes an RNA nanostructure with MG, PP7, TAR and S1m RNA aptamers. Transcription via T7 RNAP.

    Depositor

    Friedrich Simmel

    Article

    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

    Insert

    RNA nanostrcutre
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7 RNAP

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PER2:Luc Plasmid

    Plasmid

    #206875

    Purpose

    Per2 promoter luciferase reporter

    Depositor

    Michelle Farkas

    Article

    Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

    Insert

    Period 2 (Per2 Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PAX6 donor plasmid

    Plasmid

    #105239

    Purpose

    Knock in H2B-GFP into the human PAX6 locus

    Depositor

    Lorenz Studer

    Article

    Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

    Insert

    PAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tn7 helper plasmid

    Plasmid

    #141161

    Purpose

    Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

    Depositor

    Shimon Bershtein

    Article

    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

    Insert

    Tn7 transposon machinery
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinose-inducible pBAD

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tn7 integration plasmid

    Plasmid

    #154134

    Purpose

    Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.

    Depositor

    Shimon Bershtein

    Article

    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

    Inserts

    left and right end of Tn7 arm
    Spectinomycin resistant gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Spectinomycin resistance gene promoter and no

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. BASU RaPID plasmid

    Plasmid

    #107250

    Purpose

    Expresses the BASU RaPID labeling protein

    Depositor

    Paul Khavari

    Article

    Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

    Insert

    BASU
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. iYFP-Parkin Plasmid

    Plasmid

    #197107

    Purpose

    Expresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.

    Depositor

    Dori Woods

    Article

    Capelluto et al Adv Biol (Weinh). 2023 Jan 18:e2200246.

    Insert

    parkinson protein 2, E3 ubiquitin protein ligase (PRKN Human)
    

    Use

    Retroviral

    Tags

    YFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Spacer Switch Plasmid

    Plasmid

    #215346

    Purpose

    Facilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.

    Depositor

    Hank Seifert

    Article

    Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

    Insert

    OpaD spacer; Chloramphenicol Resistance Cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. "AND-gate" receiver plasmid

    Plasmid

    #127676

    Purpose

    Encodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.

    Depositor

    Friedrich Simmel

    Article

    Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

    Insert

    LuxR, GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 for LuxR, pLux for GFP

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. E.coli RaPID plasmid

    Plasmid

    #107251

    Purpose

    Expresses the E.coli RaPID labeling protein

    Depositor

    Paul Khavari

    Article

    Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

    Insert

    E.coli BirA*
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH29_32 series plasmid

    Plasmid

    #120901

    Purpose

    Also known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated device

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

    Inserts

    Ec-TTL-P109
    sRNA-A targeting sequence
    RBS TIR=974 of GFPop1 gene
    GFPop1
    RBS TIR=6474 of ECF32mut gene
    ECF32mut
    T7 terminator
    resource competitor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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