We narrowed to 144 results for: primer
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Plasmid#201764PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
ExpressionYeastAvailable SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-T4
Plasmid#201767PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
ExpressionYeastAvailable SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-T3
Plasmid#201766PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
ExpressionYeastAvailable SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Syn61Δ3(ev5)
Bacterial Strain#174514PurposeRecoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1DepositorBacterial ResistanceStreptomycinAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
B95(DE3) ΔA ΔfabR ΔserB
Bacterial Strain#197655PurposeThis strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.DepositorBacterial ResistanceNoneAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGLACTE-PseudoHT51
Plasmid#99257PurposeContains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. Internal control with synthetic primer sequence for capillary electrophoresisDepositorInsertFMR1 (FMR1 Human, Synthetic)
MutationContains synthetic, non-human, primer sequence do…Available SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGZ109 (pFA6a-3FLAG-MNase-HIS3MX6)
Plasmid#70232PurposeChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA- HIS3MX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingAvailable SinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPPEM
Plasmid#162467PurposeDestination Expression vector for monocot plant primer editingDepositorInsertCas9H840A
TagsMLV reverse transcriptaseExpressionPlantMutationChange histidine 840 to AlaninePromotermaize UbiAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGZ173 (pFA6a-SL-MNase-kanMX6)
Plasmid#70234PurposeChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsertShort linker and aa 83-231 of Mnase
UseYeast genomic targetingAvailable SinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC_sgRNA
Plasmid#68710PurposeContains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyAvailable SinceSept. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGZ110 (pFA6a-3FLAG-MNase-TRP1)
Plasmid#70233PurposeChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA-Trp1 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope tagDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL3927 (pDonor(plasmid)_Pse)
Plasmid#200905PurposeEncodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.DepositorInsertMini Tn7016 (pTarget NGS)
ExpressionMammalianAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pc5139-pEGFP-H3.1-K36M
Plasmid#241430PurposeExpresses human Histone H3.1 as fusion to GFP with lysine to methionine point mutation of K36 from plasmid H3.1 using primers: forward: ACCGGTGGCGTGATGAAACCTCATCGC & reverse: GCGATGAGGTTTCATCDepositorInserthuman Histone H3.1 (H3C1 Human)
TagsEGFPExpressionMammalianMutationlysine at position 36 to methionine (K36M)PromoterCMVAvailable SinceJune 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-TO-DsRed
Plasmid#16340DepositorInsertDsRed
ExpressionMammalianMutation5'Prime Site: Not I 3'Primer Site : No…Available SinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pc2099-pEGFP-H3.1
Plasmid#241429PurposeExpresses human Histone H3.1 as fusion to GFP constructed by PCR using Primers: h3.1-cl-fw (AAGAATTCAATGGCTCGTACGAAGCAAAC) and h3.1-cl-rv (AAGCGGCCGCTGCCCTTTCCCCACGGATG) cloned into pEGFP-C1DepositorAvailable SinceJune 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
eScaf
Bacterial Strain#220921PurposecssDNA production strainDepositorBacterial ResistanceNoneSpeciesEscherichia coliAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
BL21 ΔrecBCD
Bacterial Strain#176581PurposeE. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.DepositorBacterial ResistanceNoneAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 ΔrecBCD
Bacterial Strain#176583PurposeE. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.DepositorBacterial ResistanceChloramphenicolAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
DHL708
Bacterial Strain#98417PurposeBacterial Strain with Genotype: MC4100 ∆clpPXDepositorBacterial ResistanceStreptomycinAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Syn61
Bacterial Strain#174513PurposeRecoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1DepositorBacterial ResistanceStreptomycinAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only