We narrowed to 169 results for: primer

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pET-Impact-T4gp61(I342G)

Plasmid

#228208

Purpose

Expression in BL21(DE3) of T4 primase I342G

Depositor

Stephen Benkovic

Publication

Valentine et al Biochemistry. 2001 Dec 18;40(50):15074-8

Insert

61 (61 Escherichia phage T4)

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Mutation

Mutated isoleucine 342 to glycine (I342G), improv…

Promoter

T7

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pTBY1-T4gp61/41 fusion

Plasmid

#228210

Purpose

Expression in BL21(DE3) of T4 primase and helicase fusion with 24 amino acid linker

Depositor

Stephen Benkovic

Publication

Manosas et al Nat Chem Biol. 2009 Dec;5(12):904-12. do

Insert

61/41

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)

Plasmid

#70195

Purpose

Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector

Depositor

Richard Murray

Publication

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

tetR-deGFP fusion

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pOpen-BsupolLF

Plasmid

#165563

Purpose

Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

Bsu DNA Polymerase I, Large Fragment

Use

Synthetic Biology

Available since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)

Plasmid

#70195

Purpose

Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector

Depositor

Richard Murray

Publication

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

tetR-deGFP fusion

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pABY-T2

Plasmid

#201765

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Publication

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Expression

Yeast

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pABY-T1

Plasmid

#201764

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Publication

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Expression

Yeast

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pABY-T4

Plasmid

#201767

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Publication

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Expression

Yeast

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pABY-T3

Plasmid

#201766

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Publication

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Expression

Yeast

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGLACTE-PseudoHT51

Plasmid

#99257

Purpose

Contains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. Internal control with synthetic primer sequence for capillary electrophoresis

Depositor

Karen Usdin

Publication

Hayward et al J Mol Diagn. 2017 Nov;19(6):828-835. doi

Insert

FMR1 (FMR1 Synthetic, Human)

Mutation

Contains synthetic, non-human, primer sequence do…

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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B95(DE3) ΔA ΔfabR ΔserB

Bacterial strain

#197655

Purpose

This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

Depositor

Richard Cooley, Ryan Mehl

Publication

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Bacterial resistance

None

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
Syn61Δ3(ev5)

Bacterial strain

#174514

Purpose

Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

Depositor

Jason W Chin

Publication

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Bacterial resistance

Streptomycin

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGZ109 (pFA6a-3FLAG-MNase-HIS3MX6)

Plasmid

#70232

Purpose

ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA- HIS3MX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

Depositor

Steven Henikoff

Publication

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

3xFLAG tag and aa 83-231 of Mnase

Use

Yeast genomic targeting

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGLACTE-PseudoHT51

Plasmid

#99257

Purpose

Contains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. Internal control with synthetic primer sequence for capillary electrophoresis

Depositor

Karen Usdin

Publication

Hayward et al J Mol Diagn. 2017 Nov;19(6):828-835. doi

Insert

FMR1 (FMR1 Human, Synthetic)

Mutation

Contains synthetic, non-human, primer sequence do…

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pPPEM

Plasmid

#162467

Purpose

Destination Expression vector for monocot plant primer editing

Depositor

Adam Bogdanove

Publication

Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

Insert

Cas9H840A

Tags

MLV reverse transcriptase

Expression

Plant

Mutation

Change histidine 840 to Alanine

Promoter

maize Ubi

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pGZ173 (pFA6a-SL-MNase-kanMX6)

Plasmid

#70234

Purpose

ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

Depositor

Steven Henikoff

Publication

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

Short linker and aa 83-231 of Mnase

Use

Yeast genomic targeting

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC_sgRNA

Plasmid

#68710

Purpose

Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

Depositor

Roberto Docampo

Publication

Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Available since

Sept. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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pGZ109 (pFA6a-3FLAG-MNase-HIS3MX6)

Plasmid

#70232

Purpose

ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA- HIS3MX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

Depositor

Steven Henikoff

Publication

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

3xFLAG tag and aa 83-231 of Mnase

Use

Yeast genomic targeting

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGZ110 (pFA6a-3FLAG-MNase-TRP1)

Plasmid

#70233

Purpose

ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA-Trp1 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope tag

Depositor

Steven Henikoff

Publication

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

3xFLAG tag and aa 83-231 of Mnase

Use

Yeast genomic targeting

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGZ173 (pFA6a-SL-MNase-kanMX6)

Plasmid

#70234

Purpose

ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

Depositor

Steven Henikoff

Publication

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

Short linker and aa 83-231 of Mnase

Use

Yeast genomic targeting

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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