We narrowed to 32,137 results for: uros

1. MSCV_Cas9_puro

   Plasmid

   #65655

   Purpose

   Retroviral introduction of Cas9 into mammalian cell line.

   Depositor

   Christopher Vakoc

   Article

   Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   5 ' FLAG tag

   Expression

   Mammalian

   Promoter

   MSSV_LTR

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEFBOS_CreIRESpuro

   Plasmid

   #183812

   Purpose

   Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

   Depositor

   Ed Stanley

   Article

   Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

   Insert

   Cre recombinase
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ubc.Luc.IRES.Puro

   Plasmid

   #33307

   Depositor

   Linzhao Cheng

   Article

   Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   Ubc

   Available Since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE_puro

   Plasmid

   #118692

   Purpose

   Empty vector to express overexpression cDNA

   Depositor

   Christopher Vakoc

   Article

   Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

   Type

   Empty backbone

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAK_lentiguide_DR30_Puro

   Plasmid

   #134839

   Purpose

   Lentiviral expression CasRx guideRNA

   Depositor

   Michael Yaffe

   Insert

   CasRx guideRNA backbone
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB_iASCL1_P2A_DLX2_T2A_mCherry_Puro

   Plasmid

   #168806

   Purpose

   PiggyBac transposon construct with doxycycline inducible ASCL1, DLX2 + mCherry expression

   Depositor

   Prashant Mali

   Article

   Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

   Insert

   ASCL1-P2A-DLX2-T2A-mCherry
   

   Use

   Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe_puro_DEST_Flag_SKIL

   Plasmid

   #45265

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   SNON (SKIL Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

8. CROP_C9_Puro_PDGFRA

   Plasmid

   #183313

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets PDGFRA gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   PDGFRA
   

   Use

   Lentiviral

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

   Plasmid

   #92099

   Purpose

   Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR and TALEN; Zfn

   Expression

   Mammalian

   Promoter

   Tet-On 3G Bidirectional

   Available Since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. LRG2.1_Puro

    Plasmid

    #125594

    Purpose

    Lentiviral expression of sgRNA with GFP and puromycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter for sgRNA expression and EFS promoter…

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCoPURO

    Plasmid

    #17533

    Depositor

    Francis Castellino

    Article

    Iwaki et al Biotechniques. 2003 Sep . 35(3):482-4, 4

    Insert

    Puromycine Acetyl Transferase
    

    Expression

    Insect

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag_HsIRE1a_pBabePuro

    Plasmid

    #54337

    Purpose

    N-terminally FLAG tagged human IRE1a in retroviral pBabe puro vector

    Depositor

    David Ron

    Article

    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

    Insert

    IRE1a (ERN1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and preprotrypsin signal sequence

    Expression

    Mammalian

    Mutation

    The endogenous IRE1a signal sequence (aa1-18) was…

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAK_DR30_CasRx_Puro

    Plasmid

    #134845

    Purpose

    All in one overexpression of CasRx and guideRNA

    Depositor

    Michael Yaffe

    Insert

    RfxCas13d
    

    Use

    CRISPR

    Tags

    T2A Puromycin

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV_TRET_hNgn2_UBC_Puro

    Plasmid

    #61474

    Purpose

    3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection marker

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Inserts

    Ngn2 (NEUROG2 Human)
    Puro
    

    Use

    Lentiviral

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB_iETV2_P2A_GFP_Puro

    Plasmid

    #168805

    Purpose

    PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    ETV2 (ETV2 Human, Synthetic)
    

    Use

    Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_P2A_Puro

    Plasmid

    #211364

    Purpose

    Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

    Depositor

    Jacques Neefjes

    Article

    Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CROP_C9_Puro_PDGFRB

    Plasmid

    #183314

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets PDGFRB gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    PDGFRB
    

    Use

    Lentiviral

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB_iMYOD1_P2A_GFP_Puro

    Plasmid

    #168804

    Purpose

    PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    MYOD1 (MYOD1 Human, Synthetic)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CROP_C9_Puro_ERBB2

    Plasmid

    #183287

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets ERBB2 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    ERBB2
    

    Use

    Lentiviral

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. EF1a_Puro_Telo_v2

    Plasmid

    #195139

    Purpose

    ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Telomere (RTEL1 Human)
    

    Expression

    Mammalian

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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