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Plasmid#118692PurposeEmpty vector to express overexpression cDNADepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only
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pLentiCRISPR_Puro_sgTP53
Plasmid#187819PurposepLentiCRISPR that is selectable with puromycin with an sgRNA targeting TP53DepositorInsertsgTP53
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_hACE2_PURO
Plasmid#155295PurposeLentiviral vector to generate hACE2 stable expressing cell lineDepositorInserthACE2 (ACE2 Human)
UseLentiviralTagsC9ExpressionMutationPromoterCMVAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGH224_sgRNA_2xMS2_Puro
Plasmid#85413PurposehU6 driven sgRNA vector with 2xMS2 vectors with puro selectable markerDepositorInsertpuromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Cas9_puro
Plasmid#108100PurposeLentiviral expression plasmid of spCas9 with puromycin resistance geneDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV_TRET_hNgn2_UBC_Puro
Plasmid#61474Purpose3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection markerDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ubc.Luc.IRES.Puro
Plasmid#33307DepositorInsertFirefly Luciferase
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterUbcAvailable sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_EGFP_Donor
Plasmid#178088PurposeHDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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EF1a_Puro_Telo_v2
Plasmid#195139Purpose~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss inductionDepositorInsertTelomere (RTEL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_PGK1_3xFLAG_Twin_Strep
Plasmid#68375PurposeDoyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and TALEN; ZfnTags3xFLAG_Twin_StrepExpressionMammalianMutationPromoterhuman PGK1Available sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF1a_Puro_Telo_v1
Plasmid#195138Purpose~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss inductionDepositorInsertTelomere (RTEL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB_iMYOD1_P2A_GFP_Puro
Plasmid#168804PurposePiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expressionDepositorInsertMYOD1 (MYOD1 Human, Synthetic)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP_a_tubulin_IRES_puro2b
Plasmid#21042DepositorInsertalpha Tubulin (TUBA1B Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
Plasmid#176311PurposeExpresses NLS-pMag-CreC60 with tagBFP markerDepositorInserttagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
UseLentiviral and Synthetic BiologyTagstagBFPExpressionMammalianMutationPromoterhuman EF1alphaAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.DEST.puro
Plasmid#32682PurposeLentiviral cDNA/shRNA gateway destination vectorsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_VPR_Puro
Plasmid#167895PurposePiggyBac compatible plasmid expressing Cas9-VPRDepositorInsertCas9-VPR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_ANKRD1_KO_Puro
Plasmid#186667PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Puromycin (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabepuroBAF180
Plasmid#41078DepositorInsertBAF180 (PBRM1 Human)
UseRetroviralTagsExpressionMammalianMutation3 silent mutations: 3595 a to g; 4981 a to t; 587…PromoterAvailable sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Puro
Plasmid#186670PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Puromycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only