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MLM3613

Plasmid

#42251

Purpose

Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

Streptococcus pyogenes Cas9

Use

CRISPR; Zebrafish expression

Promoter

CMV

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2(+)-zGrad

Plasmid

#119716

Purpose

Plasmid to generate zGrad mRNA to inactivate proteins in zebrafish

Depositor

Holger Knaut

Article

Yamaguchi et al Elife. 2019 Feb 8;8. pii: 43125. doi: 10

Insert

zGrad

Expression

Bacterial

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pTol-Promoter:NLS-tdMCP-eGFP

Plasmid

#86248

Purpose

For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Insert

beta Actin promoter:NLS-tdMCPeGFP

Use

Used with transposase to make transient or stable…

Tags

NLS-tdMCP-eGFP

Promoter

Beta Actin

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST mfap4:turquoise2

Plasmid

#135218

Purpose

Cyan tagged macrophages

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

mfap4 promoter and mTurquoise2

Use

Zebrafish expression

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)

Plasmid

#92400

Purpose

Constitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and membrane tag

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKHR7

Plasmid

#74624

Purpose

a cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.

Depositor

David Grunwald

Article

Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

Insert

cmlc2::mCherry

Use

Zebrafish expression

Promoter

cmlc2

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST mfap4:tdTomato

Plasmid

#135214

Purpose

Red tagged macrophages

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

mfap4 promoter and tdTomato

Use

Zebrafish expression

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR - CaSR-EGFP

Plasmid

#191503

Purpose

A zebrafish CaSR-EGFP construct used for Gateway cloning.

Depositor

Michael Granato

Article

Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

Insert

CaSR-EGFP-SV40

Use

Zebrafish expression

Promoter

none

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-GG

Plasmid

#182154

Purpose

Expression of artificial intron separated eGFP

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

GG

Use

Zebrafish expression

Promoter

eab2

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-AA

Plasmid

#182153

Purpose

Expression of artificial intron separated mApple

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

AA

Use

Zebrafish expression

Promoter

eab2

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-AG

Plasmid

#182155

Purpose

Expression of chimeric AG (N-mApple-intron-C-eGFP)

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

AG

Use

Zebrafish expression

Promoter

eab2

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-GA

Plasmid

#182156

Purpose

Expression of chimeric GA (N-eGFP-intron-C-mApple)

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

GA

Use

Zebrafish expression

Promoter

eab2

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pSP64T-ythdf2-3xflag

Plasmid

#164489

Purpose

Expresses zebrafish ythdf2 with 3x flag tag, for mRNA injection

Depositor

Antonio Giraldez

Article

Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

Insert

ythdf2 (ythdf2 Zebrafish)

Use

Production of mrna for zebrafish injection.

Tags

3x-flagtag

Promoter

SP6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ dgat1b-FLAG

Plasmid

#232144

Purpose

Expresses zebrafish dgat1b with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat1b

Use

Zebrafish expression

Tags

FLAG

Promoter

CMV

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ mogat3b-FLAG

Plasmid

#232145

Purpose

Expresses zebrafish mogat3b with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

mogat3b

Use

Zebrafish expression

Tags

FLAG

Promoter

CMV

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-OXER1-mKate2

Plasmid

#226713

Purpose

overexpression red fluorescent OXER1 protein in zebrafish larvae

Depositor

Philipp Niethammer

Article

Ma et al Cell Rep. 2023 Jan 31;42(1):111974. doi:

Insert

OXER1 (OXER1 Human)

Use

Unspecified; Zebrafish expression

Tags

mKate2

Promoter

SP6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSP64T-ythdf1-3xflag

Plasmid

#164488

Purpose

Expresses zebrafish ythdf1 with 3x flag tag, for mRNA injection

Depositor

Antonio Giraldez

Article

Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

Insert

ythdf1 (ythdf1 Zebrafish)

Use

Production of mrna for zebrafish injection.

Tags

3x-flagtag

Promoter

SP6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSP64T-ythdf3-3xflag

Plasmid

#164490

Purpose

Expresses zebrafish ythdf3 with 3x flag tag, for mRNA injection

Depositor

Antonio Giraldez

Article

Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

Insert

ythdf3 (ythdf3 Zebrafish)

Use

Production of mrna for zebrafish injection.

Tags

3x-flagtag

Promoter

SP6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-zfTpo

Plasmid

#168198

Purpose

Express zebrafish thrombopoietin (Tpo), mRNA synthesis

Depositor

Petr Bartunek

Article

Svoboda et al Blood. 2014 Jul 10;124(2):220-8. doi: 10

Insert

Thrombopoietin (thpo Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-CASK [K56A/50R] (Recombinant Antibody)

Antibody

#183002-rAb

Purpose

Anti-CASK (Human) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Recommended Applications

Immunohistochemistry and Western Blot

Reactivity

Frog, Human, Mouse, Rat, and Zebrafish

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available Since

May 31, 2022

Availability

Industry, Academic Institutions, and Nonprofits