-
Plasmid#47584DepositorInsertpMCA2z/b (ATP2B2 Human)
UseTagsEGFP-HA-HisExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG eGFP-Oct4
Plasmid#203789PurposeCompetitive assay of Oct4 and Oct6 binding to DNA motifs.DepositorInsertPou5f1 (Pou5f1 Mouse)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.2
Plasmid#88999PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 1.2
Plasmid#89000PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.1
Plasmid#88994PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.1 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 1.1
Plasmid#88997PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.1 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- WTN23C11orf83-GFP
Plasmid#65845PurposeMammalian expression of the wild type N terminal part (N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)DepositorInsertTransmembrane (23 AA) of UQCC3 (UQCC3 Human)
UseTagsGFPExpressionMammalianMutationWT TransmembranePromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF-DEST51 GFP-PNRC1
Plasmid#123295PurposeMammalian expression of PNRC1 N-GFPDepositorInsertPNRC1 (PNRC1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG eGFP-Oct6
Plasmid#203790PurposeCompetitive assay of Oct4 and Oct6 binding to DNA motifs.DepositorInsertPou3f1 (Pou3f1 Mouse)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
myc-Miro1 (lacking TM)
Plasmid#127613PurposeHuman Miro1 with the transmembrane deletedDepositorInsertMiro1 (RHOT1 Human)
UseTagsmycExpressionMammalianMutationDeletion of transmembrane domainPromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-GFP-Pho8-406
Plasmid#207006PurposeIntegration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.DepositorInsertPHO8
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
ptAD-seq-ubi63E-Gal4-DBD
Plasmid#111930PurposeVector to express tAD candidates from a library cloned from fragmented transcription factor coding sequences fused to the Gal4 DNA binding domain.DepositorInserttAD-seq Gal4-DBD
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFASTbac-HisGST-PTPIP51(36-470)
Plasmid#170531PurposeExpresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9DepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 )
UseTagsGST tag and His tagExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti4 Blast RFP-PNRC1
Plasmid#123297PurposeMammalian expression of PNRC1 N-RFPDepositorInsertPNRC1 (PNRC1 Human)
UseLentiviralTagsTurboRFPExpressionMammalianMutationPromoterAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF-DEST51 PNRC1
Plasmid#123293PurposeExpression of PNRC1 in mammalian cellsDepositorInsertPNRC1 (PNRC1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH_CMV_TFAM_mKate2
Plasmid#167331PurposeExpresses mKate2 fused to full length TFAM in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_blast_MCS_Nard_GFP_LAMIN
Plasmid#167340PurposeExpresses GFP fused to lamin A (LA) in mammalian cellsDepositorInsertLamin A (LMNA Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPLICS NU-MT P2A
Plasmid#210129PurposeDetect the Nucleus-Mitochondria contactDepositorInsertSPLICS NU-MT P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-puro-PTPIP51(1-470)
Plasmid#170536PurposeExpresses PTPIP51(1-470) in human HeLa cells after transfectionDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterCAG promoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-DEST
Plasmid#119745PurposeAllows Gateway cloning of gene of interest into retroviral pMSCV vector (with Puromycin resistance)DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only