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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,439 results for: hal.2

Showing: 381 - 400 of 2439 results

  1. R620-M19-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417T/E484K/N501Y

    Plasmid
    #170203
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Tags
    3C-His8-SBP
    Expression
    Mammalian
    Mutation
    K417T, E484K, N501Y
    Promoter
    CMV51
    Available Since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170203-plasmid-map-sequence-id-334219

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  2. R620-M05-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 K417N/E484K/N501Y

    Plasmid
    #170191
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Tags
    His6
    Expression
    Mammalian
    Mutation
    K417N, E484K, N501Y
    Promoter
    CMV51
    Available Since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170191-plasmid-map-sequence-id-334254

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  3. pAc5.1B-lambdaN-HA-DmMTR-4_AA

    Plasmid
    #148215
    Purpose
    Insect Expression of DmMTR-4
    Depositor
    Elisa Izaurralde
    Insert
    DmMTR-4 (l(2)35Df Fly)
    Expression
    Insect
    Mutation
    7 silent mutations compared to the sequence given…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148215-plasmid-map-sequence-id-290663

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  4. pAc5.1B-EGFP-DmMTR-4

    Plasmid
    #66591
    Purpose
    expresses EGFP-tagged MTR-4 in Drosophila cells
    Depositor
    Elisa Izaurralde
    Insert
    MTR-4 (l(2)35Df Fly)
    Use
    Drosophila expression
    Tags
    EGFP
    Promoter
    Actin 5C
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    66591-plasmid-map-sequence-id-113866

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  13. LB101: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#2 gRNA

    Plasmid
    #131758
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    TRE#2 SaCas9 gRNA
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Promoter
    hU6
    Available Since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    131758-plasmid-map-sequence-id-314395

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  16. R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351

    Plasmid
    #170196
    Purpose
    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)
    Depositor
    Dominic Esposito
    Article
    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568
    Insert
    SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
    Tags
    3C-His8-Strep2x2
    Expression
    Mammalian
    Mutation
    L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…
    Promoter
    CMV51
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    170196-plasmid-map-sequence-id-334281

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  17. R620-M13-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.1.7

    Plasmid
    #170197
    Purpose
    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant)
    Depositor
    Dominic Esposito
    Article
    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568
    Insert
    SARS-CoV-2 S (spike) B.1.1.7 (S SARS-CoV-2)
    Tags
    His6
    Expression
    Mammalian
    Mutation
    Δ(69-70), Δ144, N501Y, A570D, D614G, P681H, T716I…
    Promoter
    CMV51
    Available Since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    170197-plasmid-map-sequence-id-334257

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  18. R620-M14-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.351

    Plasmid
    #170198
    Purpose
    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)
    Depositor
    Dominic Esposito
    Article
    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568
    Insert
    SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
    Tags
    His6
    Expression
    Mammalian
    Mutation
    L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…
    Promoter
    CMV51
    Available Since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170198-plasmid-map-sequence-id-334260

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  19. R620-M01-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.1.7

    Plasmid
    #170188
    Purpose
    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant)
    Depositor
    Dominic Esposito
    Article
    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568
    Insert
    SARS-CoV-2 S (spike) B.1.1.7 (S SARS-CoV-2)
    Tags
    3C-His8-Strep2x2
    Expression
    Mammalian
    Mutation
    Δ(69-70), Δ144, N501Y, A570D, D614G, P681H, T716I…
    Promoter
    CMV51
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    170188-plasmid-map-sequence-id-334256

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Showing: 381 - 400 of 2439 results