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Plasmid#182679PurposeExpression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.DepositorInsertNefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
UseTagsExpressionMammalianMutationPromoterU6 and chicken beta-actin promoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Tubb3-2xGFP KI
Plasmid#183443PurposeGFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)DepositorInsertgRNA and 2xGFP donor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Tubb3-3xGFP KI
Plasmid#183444PurposeGFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)DepositorInsertgRNA and 3xGFP donor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPD152 Reporter Template v2
Plasmid#138752PurposeTemplate for COMET promoters, expresses EYFP reporter upon promoter activation. BsaI sites produce palindromic sticky ends.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Donor/Reporter-COL4A3
Plasmid#130279PurposeCOL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor templateDepositorInsertmCherry-eGFP
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-EpoRtm
Plasmid#114286PurposeTET-inducible expression of Erythropoietin receptor without cytoplasmic tailDepositorInsertErythropoietin Receptor (Epor Rat)
UseLentiviralTagsExpressionMutationdeletion of the cytoplasmic domainPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiR-report-hARPC2
Plasmid#112549Purpose3'UTR reporter assayDepositorInsertARPC2 (ARPC2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ternary BAC Reporter
Plasmid#78220Purposesynthetic gene circuitDepositorInsertsynthetic gene circuit
UseTagsExpressionMutationPromoterAvailable sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMIR-192-Reporter
Plasmid#71872PurposeMammalian expression vector for the analysis of miR-19α activityDepositorInsertReverse complementary sequence of miR-192
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIR-193a-5p-Reporter
Plasmid#71873PurposeMammalian expression vector for the analysis of miR-193a-5p activityDepositorInsertReverse complementary sequence of miR-193a-5p
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_pNeurog2-Luciferase reporter
Plasmid#64160PurposePhotoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.DepositorInsertsgRNA1 for pNeurog2-Luciferase reporter
UseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
ZE49 pSPORT1-Sp5-l
Plasmid#17090DepositorInsertSp5l (sp5l Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SF3B1-K700E-reporter
Plasmid#200318PurposeA fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.DepositorInsertBSD-2A-EGFPi
UseLentiviralTagsExpressionMammalianMutationPromoterEF1-aAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFEB promoter-luciferase reporter
Plasmid#66801PurposeReporter for activity of TFEB gene promoterDepositorInsertTFEB promoter (Tfeb Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
HIF-TRE-mStrawberry reporter
Plasmid#158681PurposeThis plasmid is a HIF1A pathway reporter. It has a 6 x HIF binding element promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-TOP-dGFP-reporter
Plasmid#14715PurposeBeta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).DepositorInsertLEF-1/TCF-responsive promoter (Ctnnb1 )
UseLentiviralTagsd2 eGFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
AKT-translocation-mStrawberry reporter
Plasmid#158685PurposeThis plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
p53-TRE-mStrawberry reporter
Plasmid#158684PurposeThis plasmid is a p53 pathway reporter. It has two copies of wild-type p53 binding sites promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Cloning template vector
Plasmid#131471PurposeCloning template form ORANGE method based knock-in constructsDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible Luciferase reporter
Plasmid#64127PurposePhotoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.DepositorInsertluciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterTetOx13-CMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only