Skip to main content
Addgene
Showing: 5971 - 6000 of 8427 results
  1. pTFE3-donor

    Plasmid
    #112377
    Purpose
    CRISPR donor plasmid to tag human transcription factor TFE3 with GFP
    Depositor
    Insert
    TFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pFLIP38

    Plasmid
    #65616
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    6His-edCit-attR1 and attR2-edCer-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. GGE252

    Plasmid
    #120791
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Insert
    InsDown_GSY-NotI
    Use
    Cloning/assembly plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. GGE253

    Plasmid
    #120790
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Insert
    InsUp_GSY-NotI
    Use
    Cloning/assembly plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pFLIP43

    Plasmid
    #65618
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    6His-edAFPt9-attR1 and attR2-edCer-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. pJUNB-donor

    Plasmid
    #112313
    Purpose
    CRISPR donor plasmid to tag human transcription factor JUNB with GFP
    Depositor
    Insert
    JUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:19:12792992:C>G, hg38:19:12793018:T>G,…
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. pHEY1-donor

    Plasmid
    #112297
    Purpose
    CRISPR donor plasmid to tag human transcription factor HEY1 with GFP
    Depositor
    Insert
    HEY1 homology arms flanking EGFP-IRES-Neo cassette (HEY1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:8:79765311:G>A
    Promoter
    Available since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. GGE209

    Plasmid
    #120793
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Insert
    InsUp-Mfe
    Use
    Cloning/assembly plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pFLIP37

    Plasmid
    #65505
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    6His-Cit-attR1 and attR2-Cer-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
  10. 5VC8 (WHSC1)

    Plasmid
    #101262
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    WHSC1 (NSD2 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 2, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
  11. pAC575

    Plasmid
    #127976
    Purpose
    AMA1 plasmid with ble selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Insert
    ble (bleomycin resistance marker)
    Use
    Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
  12. pDLX1-donor

    Plasmid
    #112343
    Purpose
    CRISPR donor plasmid to tag human transcription factor DLX1 with GFP
    Depositor
    Insert
    DLX1 homology arms flanking EGFP-IRES-Neo cassette (DLX1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:2:172087403:G>C,hg38:2:172087433:G>A,h…
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. pVYD

    Plasmid
    #61302
    Purpose
    Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to SYFP2
    Depositor
    Type
    Empty backbone
    Use
    Tags
    SYFP2
    Expression
    Bacterial
    Mutation
    Promoter
    PV10
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
  14. pICSL70005

    Plasmid
    #50335
    Depositor
    Insert
    bialaphos resistance cassette (Nos promoter (A. tumefaciens) + phosphinothricin acetyl transferase (S. hygroscopicus) + Nos term
    Use
    Plant expression
    Tags
    Expression
    Mutation
    BsaI/BbsI sites removed by point-mutation
    Promoter
    Available since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 5971 - 6000 of 8427 results