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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 4,425 results for: ARA-2

Showing: 3981 - 4000 of 4425 results

  3. pHis6-SUMO-g0s2

    Plasmid
    #179293
    Purpose
    E. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do
    Insert
    G0S2 (G0S2 Human)
    Use
    Tags
    His6 + SUMO + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    full-length G0S2, residues 1-103 from accession n…
    Promoter
    T7
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179293-plasmid-map-sequence-id-354916

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  4. MBP-g0s2

    Plasmid
    #179294
    Purpose
    E. coli expression plasmid (Ptac promoter) for full-length human G0S2 with N-terminal mature maltose binding protein + enterokinase cleavage site
    Depositor
    Debra Hansen
    Article
    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do
    Insert
    G0S2 (G0S2 Human)
    Use
    Tags
    mature maltose binding protein + enterokinase cle…
    Expression
    Bacterial
    Mutation
    full-length G0S2, residues 1-103 from accession n…
    Promoter
    Ptac
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179294-plasmid-map-sequence-id-354917

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  5. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119(SpeI)
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221135-plasmid-map-sequence-id-436077

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  12. pJR85

    Plasmid
    #140095
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140095-plasmid-map-sequence-id-292013

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  13. FH-Tet2-pEF

    Plasmid
    #41710
    Purpose
    This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system
    Depositor
    Anjana Rao
    Article
    Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi
    Insert
    tet methylcytosine dioxygenase 2 (Tet2 Mouse)
    Use
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    41710-plasmid-map-sequence-id-322448

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Showing: 3981 - 4000 of 4425 results