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pBEL2062

Plasmid

#162148

Purpose

Expression plasmid for MlaE Leu70Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Leu70Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2063

Plasmid

#162149

Purpose

Expression plasmid for MlaE Leu99Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Leu99Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2065

Plasmid

#162150

Purpose

Expression plasmid for MlaE Trp149Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Trp149Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2066

Plasmid

#162151

Purpose

Expression plasmid for MlaE Phe209Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Phe209Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2139

Plasmid

#162132

Purpose

Expression and Complementation plasmid for MlaD TM swap with LptC TM

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(TMdeltaLptC TM)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2138

Plasmid

#162133

Purpose

Expression and Complementation plasmid for MlaD TM swap with LetB TM

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(TMdeltaLetB TM)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL1198

Plasmid

#162134

Purpose

Expression and complementation plasmid for MlaFEDCB, with N-terminal his tag on MlaE

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2093

Plasmid

#162135

Purpose

Expression and Complementation plasmid for MlaE IF1 1-15 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-15)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2132

Plasmid

#162136

Purpose

Expression and Complementation plasmid for MlaE IF1 1-25 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-25)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2092

Plasmid

#162137

Purpose

Expression and Complementation plasmid for MlaE IF1 1-30 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-30)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2133

Plasmid

#162138

Purpose

Expression and Complementation plasmid for MlaE IF1 1-39 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-39)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2099

Plasmid

#162139

Purpose

Complementation plasmid for MlaE Y81A

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(Y81A)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2100

Plasmid

#162140

Purpose

Complementation plasmid for MlaE Y81W

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(Y81W)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2098

Plasmid

#162141

Purpose

Complementation plasmid for MlaE R97A

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(R97A)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAB166

Plasmid

#101668

Purpose

Opto-T7RNAP*(563-F2), half expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F2), half expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB171

Plasmid

#101670

Purpose

Opto-T7RNAP*(69), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(69), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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LDB34

Plasmid

#185431

Purpose

Yeast expression of Nup1 C-terminal truncation (nup1-21 allele)

Depositor

Ken Belanger, Laura Davis

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Mutation

Truncation at amino acid 1041 from the C-term of …

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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KBB11

Plasmid

#185422

Purpose

Full-length KAP60/SRP1

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi

Insert

KAP60

Expression

Yeast

Promoter

endogenous

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB33

Plasmid

#185430

Purpose

Yeast expression of Nup1 N-terminal truncation (nup1-15 allele)

Depositor

Ken Belanger, Laura Davis

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Mutation

Truncation of amino acids 3-191 from the N-term o…

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-SpBeta-catenin

Plasmid

#185579

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

Beta-catenin

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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