We narrowed to 13,471 results for: Mpl;

1. pcDNA3.1_hMed25

   Plasmid

   #206958

   Purpose

   The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.

   Depositor

   Catharine Ross, Reza Zolfaghari

   Insert

   Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas1_Cas2/3

   Plasmid

   #89240

   Purpose

   Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1

   Depositor

   Blake Wiedenheft

   Article

   Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

   Insert

   Cas1 and Cas2/3
   

   Tags

   Strep II tag on Cas1

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. PROM5_35Sx2

   Plasmid

   #136112

   Purpose

   Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   PROM_35Sx2 (CaMV)
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. PROM5_35S

   Plasmid

   #136111

   Purpose

   Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   PROM_35S (CaMV)
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. KBB364

   Plasmid

   #185083

   Purpose

   RFA1 fused to GFP under control of GAL1 promoter

   Depositor

   Ken Belanger

   Article

   Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

   Insert

   RFA1
   

   Tags

   GFP

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-NPM NESM

   Plasmid

   #13282

   Depositor

   Xin Wang

   Article

   Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

   Insert

   NPM (NPM1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   L100A, L102A

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pscALPS gag-gfp Blasti

   Plasmid

   #115803

   Purpose

   Express gag-gfp fusion driven by SFFV promoter

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   gag-gfp
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSG424 Smad2 (Gal4-Smad2)

   Plasmid

   #14932

   Depositor

   Joan Massague

   Article

   Liu et al Genes Dev. 1997 Dec 1. 11(23):3157-67.

   Insert

   Smad2 (SMAD2 Human)
   

   Tags

   Gal4

   Expression

   Mammalian

   Available Since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

9. L1_KanR-Ck1

   Plasmid

   #136123

   Purpose

   L1 in position 1 (next to LB in L2 construct), for KanR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpEF1a:nptII_3t35S
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCk1_spacer

    Plasmid

    #136071

    Purpose

    Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAd-Track SIRT1

    Plasmid

    #8440

    Depositor

    Pere Puigserver

    Article

    Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Adenoviral

    Expression

    Mammalian

    Available Since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 FLAG-SIV-RCM-vpx

    Plasmid

    #115844

    Purpose

    Encodes codon optimized FLAG tagged SIVRCM-Vpx

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVRCM-Vpx
    

    Tags

    3x-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEM-T Easy-AtpU6-26

    Plasmid

    #160218

    Purpose

    AtpU6-26 Golden Gate level 0 piece to express gRNAs

    Depositor

    Matias Kirst

    Article

    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

    Insert

    pAtU6-26 promoter
    

    Use

    Golden gate level 0 piece to express grnas

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-NPM NLS1D

    Plasmid

    #13284

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of aa134-aa142

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pMaCTag-Z21

    Plasmid

    #120064

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 24xSunTag

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    24xSunTag
    

    Use

    Pcr template

    Tags

    24xSunTag

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBABE M33 flag

    Plasmid

    #1952

    Depositor

    Robert Kingston

    Article

    Levine et al Mol Cell Biol 2002 Sep;22(17):6070-8.

    Insert

    M33 (Cbx2 Mouse)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pMBPCas2/3

    Plasmid

    #89238

    Purpose

    Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

    Depositor

    Blake Wiedenheft

    Article

    Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

    Insert

    Cas2/3
    

    Tags

    Strep II-MBP

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Actin-53a-ires-EGFP

    Plasmid

    #25460

    Depositor

    Jerry Crabtree

    Article

    Wu et al Neuron. 2007 Oct 4. 56(1):94-108.

    Insert

    BAF53a (Actl6a Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Sept. 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. YSBE 517 : pNEO_6xHA

    Plasmid

    #92083

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, Neo selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    6xHA
    

    Use

    tagging proteins in cryptococcus neoforman

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-beta-hRLuc-V5-6xbsMS2-MALAT_X

    Plasmid

    #147924

    Purpose

    Mammalian Expression of 6xbsMS2-Malat

    Depositor

    Elisa Izaurralde

    Article

    Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

    Insert

    6xbsMS2-Malat (Malat1 Mouse)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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