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pCEP4-myc-HLA-A2-VN (Y84P)

Plasmid

#135495

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y84P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Y84P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (E166P)

Plasmid

#135498

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (E166P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

E166P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-KAT14-547-782

Plasmid

#118376

Purpose

Gateway compatible donor vector with KAT14-547-782 truncation.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

KAT14 (KAT14 Human)

Use

Donor-plasmid

Mutation

Truncation (aa 547-782)

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-TET1-878-911-I894A(pL84)

Plasmid

#124404

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with I894A mutation.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911(I894A mutation) (TET1 Human)

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

I894A

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-TET1-878-911-T898A(pL84)

Plasmid

#124403

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911(T898A mutation) (TET1 Human)

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

T898A

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-TET1-878-911-L900A(pL84)

Plasmid

#124402

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911(L900A mutation) (TET1 Human)

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

L900A

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-TET1-878-911-L897A(pL84)

Plasmid

#124401

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L897A mutation.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911(L897A mutation) (TET1 Human)

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

L897A

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-KAT14-1-546

Plasmid

#118377

Purpose

Gateway compatible donor vector with KAT14-1-546 truncation.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

KAT14 (KAT14 Human)

Use

Donor-plasmid

Mutation

Truncation (aa 1-546)

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCIneoRL-Notch1 3'UTR-mir200c-mut

Plasmid

#84596

Purpose

Renilla Luciferase reporter assay for NOTCH1 3'UTR with mutations on miR-200c binding site

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

NOTCH1 3'UTR (NOTCH1 Human)

Use

Luciferase

Tags

Renilla Luciferase

Mutation

Mutation on miR-200c binding site on NOTCH1 3…

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only
pCIneoRL-LFNG 3'UTR-mir200c-mut

Plasmid

#84594

Purpose

Renilla Luciferase reporter assay for LFNG 3'UTR with mutations on miR-200c binding site

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

LFNG 3'UTR (LFNG Human)

Use

Luciferase

Tags

Renilla Luciferase

Mutation

Mutation on miR-200c binding site on LFNG 3'…

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only
KLHL31 pgk (1088)

Plasmid

#62031

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

KLHL31 (Klhl31 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pZS1[GalS-L,RbsR-L]

Plasmid

#60763

Purpose

Contains PIq driving expression GalS-L, and PIq driving expression of RbsR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
RbsR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with GalS L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-L,FruR-L]

Plasmid

#60764

Purpose

Contains PIq driving expression GalS-L, and PIq driving expression of FruR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
FruR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with FruR L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-L,TreR-L]

Plasmid

#60765

Purpose

Contains PIq driving expression GalS-L, and PI driving expression of TreR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
TreR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with GalS L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[FruR-L,RbsR-L]

Plasmid

#60761

Purpose

Contains PIq driving expression TreR-L, and PIq driving expression of RbsR-L

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

FruR-L
RbsR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with FruR L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[RbsR-L,TreR-L]

Plasmid

#60766

Purpose

Contains PIq driving expression RbsR-L, and PI driving expression of TreR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

RbsR-L
TreR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with RbsR L…

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCE-mp53DD

Plasmid

#41856

Purpose

Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

Trp53 (Trp53 Mouse)

Expression

Mammalian

Mutation

Δ40-903

Promoter

CAG

Available Since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1(nsp8-nsp7)(nsp12)

Plasmid

#165451

Purpose

Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

Depositor

Marc Delarue

Article

Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

Inserts

nsp12
nsp8
nsp7

Tags

14Histidine-TEV

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCE-hUL

Plasmid

#41855

Purpose

Non-integrating (episomal) expression of human L-MYC and LIN28

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

L-MYC, LIN28 (MYCL, LIN28A Human)

Expression

Mammalian

Promoter

CAG

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCXLE-mp53DD

Plasmid

#41859

Purpose

Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

Trp 53 (Trp53 Mouse)

Expression

Mammalian

Mutation

Δ40-903

Promoter

CAG

Available Since

July 17, 2013

Availability

Academic Institutions and Nonprofits only

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