Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 14,067 results for: LEA

Clear keywords
Showing: 4021 - 4040 of 14067 results

  1. pGBW-m4046758

    Plasmid
    #145670
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    145670-plasmid-map-sequence-id-297837

    Enlarge

  2. pGBW-m4046731

    Plasmid
    #145633
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp2
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145633-plasmid-map-sequence-id-292494

    Enlarge

  8. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS

    Plasmid
    #107319
    Purpose
    Expresses SpCas9 fused to dSaCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SaCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 is fused to nuclease dead SaCas9 (D10A, N5…
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107319-plasmid-map-sequence-id-204682

    Enlarge

  16. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

    Plasmid
    #87827
    Purpose
    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Publication
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    NLS
    PTS1
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87827-plasmid-map-sequence-id-175008

    Enlarge

Showing: 4021 - 4040 of 14067 results