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AB.pCCL.sin.cPPT.U6.miR-27b-Decoy.hPGK.GFP.WPRE

Plasmid

#46603

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-27b Decoy (MIR27B, Mir27b Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
mIFP-Endo-14

Plasmid

#56219

Purpose

Localization: Endosomes, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Endo (RHOB Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pECE M2-SNX17 S437A

Plasmid

#69812

Purpose

Expresses M2-SNX17 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Sorting nexin-17 (SNX17 Human)

Tags

FLAG

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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mCardinal-Myotilin-C-14

Plasmid

#56169

Purpose

Localization: Cytoskeleton, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Myotilin (MYOT Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCOG75-Sem1 Sc

Plasmid

#83233

Purpose

pCDF-His6-MBP-Sem1 Sc

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

SEM 1 (SEM1 Budding Yeast)

Tags

His-mbp

Expression

Bacterial

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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WPXL SOX10 gRNA

Plasmid

#101923

Purpose

SOX10 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

Depositor

Danwei Huangfu

Article

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

SOX10 promoter targeting gRNA

Use

Lentiviral

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pLncEXP-lnc-BATE1

Plasmid

#64864

Purpose

Expresses the longest isoform of lnc-BATE1

Depositor

Lei Sun

Article

Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

Insert

lnc-BATE1

Use

Retroviral

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only
pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

Plasmid

#69232

Purpose

Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-LRTM2

Plasmid

#69237

Purpose

U6 driven SpCas9 sgRNA expression for LRTM2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

LRTM2 sgRNA (LRTM2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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mCardinal-Cx43-7

Plasmid

#56157

Purpose

Localization: Gap Junctions, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Cx43

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
tdEos-PMP-N-10

Plasmid

#57658

Purpose

Localization: Peroxisomes, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Huang et al Nat Methods. 2013 Jul;10(7):653-8. doi:

Insert

PMP (PXMP2 Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
AB.pCCL.sin.cPPT.U6.miR-139-5p-Decoy.hPGK.GFP.WPRE

Plasmid

#46588

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-139-5p Decoy (MIR139, Mir139 Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-127-Decoy.hPGK.GFP.WPRE

Plasmid

#46583

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-127 Decoy (MIR127, Mir127 Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-130b-Decoy.hPGK.GFP.WPRE

Plasmid

#46586

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-130b Decoy (MIR130B, Mir130b Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pFAB3665

Plasmid

#47832

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 1 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 1 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3701

Plasmid

#47835

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 4 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 4 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3845

Plasmid

#47840

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 9 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 9 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3857

Plasmid

#47841

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 10 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 10 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-100-Decoy.hPGK.GFP.WPRE

Plasmid

#46580

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-100 Decoy (MIR100, Mir100 Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pFAB1902

Plasmid

#47858

Purpose

BIOFAB reporter plasmid for measuring M13_central_T_linker_rrnD_T1 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

M13_central_T_linker_rrnD_T1 double terminator

Use

Synthetic Biology

Expression

Bacterial

Mutation

concatenate of M13 central and rrnD

Promoter

pLTetO13

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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