We narrowed to 4,859 results for: U6...

1. PEA1-Nuclease-GFP

   Plasmid

   #171994

   Purpose

   Delivers all prime editing nuclease components in a single, GFP selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GC to CA point mutation in SpCas9 to restore nucl…

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORANGE GFP-RAB11a KI

   Plasmid

   #131499

   Purpose

   Endogenous tagging of RAB11: N-terminal (amino acid position: before startcodon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Rab11a, Rab11a Mouse, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX462-hPRKAA2-gRNA_A

   Plasmid

   #74376

   Purpose

   gRNA_A to knockout human AMPK alpha 2 using Cas9n.

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTE4497

   Plasmid

   #80339

   Purpose

   mammalian expression FnCpf1 nuclease and Fn crRNA

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   FnCpf1
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX462-hPRKAA2-gRNA_B

   Plasmid

   #74377

   Purpose

   gRNA_B to knockout human AMPK alpha 2 using Cas9n

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-ITR-EcYtR(NGS-6)-mCherry

   Plasmid

   #217363

   Purpose

   Expresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   E. coli tyrosine tRNA mutant, "NGS-6"
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-ITR-PytR(PyOtR)-mCherry

   Plasmid

   #204870

   Purpose

   Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

   Insert

   M. mazei pyrrolysyl tRNA mutant "PyOtR"
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

   Promoter

   U6

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cbh_v5 AAV-saCBE C-terminal

   Plasmid

   #137183

   Purpose

   AAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-saCBE C-terminal
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Cbh

   Available since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. KAT2A sgRNA2

   Plasmid

   #138186

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human KAT2A

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   KAT2A (KAT2A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. KAT2A sgRNA1

    Plasmid

    #138185

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KAT2A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    KAT2A (KAT2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-sgLkb1/Cre

    Plasmid

    #66894

    Purpose

    Expresses an Lkb1-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

    Insert

    sgLkb1
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. WT1 KI gRNA2

    Plasmid

    #92313

    Purpose

    CRISPR-GFP-gRNA for cutting WT1

    Depositor

    Sean Palecek

    Article

    Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

    Insert

    WT1 (WT1 Human)
    

    Use

    Tags

    CRISPR GFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLCKOv3

    Plasmid

    #209046

    Purpose

    Lentiviral backbone for expressing U6 driven SpCas9 sgRNAs with BveI cloning sites, SpCas9 tracrRNAv1 (Dang et al., 2015; PMID: 26671237) and puromycin selection marker

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaActin binding domain

    Plasmid

    #187276

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Actin binding domain

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    deltaActin binding domain

    Promoter

    U6, CMV

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTBL680 CHYRON3 integration construct

    Plasmid

    #126448

    Purpose

    To integrate the CHYRON3 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6/3xLacO-CHYRON3 hgRNA
    pCMV-puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6/3xLacO

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO_S.NWS.VSVg_NGFR

    Plasmid

    #158348

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.NWS.VSVg

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAcBac1-Mb-Fluoro-Phe B5

    Plasmid

    #197576

    Purpose

    Expression of Methanosarcina barkeri (Mb) Fluoro-Phe B5 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10

    Inserts

    Mb Pyl Fluoro-Phe B5 synthetase
    Pyl-tRNA (4 copies)
    

    Use

    Tags

    Nuclear export sequence + FLAG

    Expression

    Mammalian

    Mutation

    N311G, C313A

    Promoter

    CMV and U6/H1

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaMyosin binding domain

    Plasmid

    #187275

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Myosin binding domain

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    deltaMyosin binding domain

    Promoter

    U6, CMV

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

    Plasmid

    #176839

    Purpose

    To recruit SAM complex to human CYP3A4 proximal enhancer.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    Human CYP3A4 proximal enhancer sgRNA for activation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJW1838

    Plasmid

    #154324

    Purpose

    sgRNA Destination Vector for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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