We narrowed to 174,954 results for: Gene

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AAVS1-Blasticidin-CAG-Flpe-ERT2

Plasmid

#68461

Purpose

AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

mammalian-codon-optimized Flpe recombinase fused with ERT2

Use

Synthetic Biology

Tags

ERT2

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCfB2904(XI-3 MarkerFree)

Plasmid

#73276

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-LMP1-IRES-GFP

Plasmid

#220350

Purpose

Expresses EBV latent gene LMP1 in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-NanoLuc-Puromycin

Plasmid

#167209

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

NanoLuc

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pH7WG2B-OsMIR390-B/c

Plasmid

#61465

Purpose

Plant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

Depositor

James Carrington

Article

Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

Insert

OsMIR390-B/c

Expression

Plant

Mutation

O. sativa MIR390 precursor sequence including a c…

Promoter

OsUBI

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mTagBFP2-Blasticidin

Plasmid

#167207

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mTagBFP2

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mCamk2a-EGFP

Plasmid

#153197

Purpose

Mouse Camk2a (mCamk2a) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt

Plasmid

#175563

Purpose

pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-1

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

GOXL1

Tags

Citrine

Expression

Plant

Promoter

RUBY

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB2909(XII-5 MarkerFree)

Plasmid

#73281

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-CAG-EGFP

Plasmid

#153186

Purpose

CAG promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDN-D2irC6kwh

Plasmid

#44725

Depositor

Gabor Balazsi

Article

Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

Inserts

pCMV-D2i promoter
mCherry

Use

Synthetic Biology; Expression regulator/reporter

Tags

Rabbit β-globin intron II and WPRE

Expression

Mammalian

Mutation

Initiator motif (Inr) displaced relative to pCMV-…

Promoter

pCMV-D2i

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pD/Paro-IFN

Plasmid

#205439

Purpose

A donor vector plasmid for Cre-mediated integration of paromomycin resistance (Paro) and dog IFNα-4 (IFNα-4) genes

Depositor

Masamichi Kamihira

Article

Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478

Inserts

dog interferon α-4 gene
paromomycin resistance gene

Use

Cre/Lox; Chlamydomonas expression

Promoter

Heat shock protein 70A (HSP70A)/ribulose bisphosp…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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PAX6 CKO 2/7 Donor plasmid

Plasmid

#68467

Purpose

donor plasmid for targeting PAX6 locus

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

FRT flanked exon4 of PAX6 gene (PAX6 Human)

Use

Cre/Lox

Expression

Mammalian

Promoter

mouse PGK promoter

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCfB3035(X-4 MarkerFree)

Plasmid

#73273

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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E12-cTAP

Plasmid

#20916

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

E12 cTAP (Tcf3 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

TAP tag is fused to the 3' end of the E12 ge…

Available Since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
pCfB3034(X-3 MarkerFree)

Plasmid

#73272

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-Zeo-TTF-1

Plasmid

#75089

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

TTF-1 (NKX2-1 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Available Since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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pDN-G1Gh

Plasmid

#44507

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
yEGFP

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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PAX6 sgRNA7

Plasmid

#68466

Purpose

targeting PAX6 gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

sgRNA targeting PAX6 (PAX6 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pFAB3833

Plasmid

#47839

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 8 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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