We narrowed to 23,628 results for: crispr

1. pMK33/Cas9

   Plasmid

   #133781

   Purpose

   Expresses drosophila-optimized Cas9

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

   Insert

   Drosophila-optimized Cas9
   

   Expression

   Insect

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLsCas13a-gRNA-NT

   Plasmid

   #164857

   Purpose

   Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   LsCas13a-gRNA-NT
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHelper_ShCAST

   Plasmid

   #127922

   Purpose

   Expresses ShCAST and a crRNA targeting PSP1

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCSh5vio-2300

   Plasmid

   #170987

   Purpose

   Encodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   Sh-CAST sgRNA 2300 violacein
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   placI

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ultra CAST

   Plasmid

   #236111

   Purpose

   Encodes sfGFP BbsI dropout for cloning in guide RNA sequence, encodes mScarlet BsmBI/BsaI dropout for cloning in cargo sequence

   Depositor

   Jeffrey Barrick

   Article

   VanDieren et al MicroPubl Biol. 2025 Aug 2;2025:10.17912

   Type

   Empty backbone

   Available Since

   July 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAL-rfp_T

   Plasmid

   #74075

   Purpose

   pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   sgRNA rfp (T)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   ptac

   Available Since

   April 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. bRA66

   Plasmid

   #100952

   Purpose

   GAL1 driven Cas9. gRNA can be cloned into BplI sites.

   Depositor

   James Haber

   Article

   Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

   Plasmid

   #174138

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   VRER=D1135V/G1218R/R1335E/T1337R

   Promoter

   CMV and T7

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. vHB16

   Plasmid

   #193455

   Purpose

   Expression of GFP-Cas9-NLS and gRNA in A. castellanii

   Depositor

   Chantal Abergel

   Article

   Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

   Insert

   tRNA-gRNA
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX851

    Plasmid

    #62883

    Purpose

    N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX852

    Plasmid

    #62884

    Purpose

    C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS SV40, and NLS nucleoplasmin

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas1+2

    Plasmid

    #72676

    Purpose

    Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

    Depositor

    Udi Qimron

    Article

    Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

    Insert

    Cas1, Cas2
    

    Expression

    Bacterial

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAH01Sp::Cas9

    Plasmid

    #128160

    Purpose

    Ptet-controlled expression of S. pyogenes Cas9

    Depositor

    Calvin Henard

    Article

    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

    Insert

    Sp Cas9
    

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJB166

    Plasmid

    #86998

    Purpose

    CRISPR/Cas9 in fission yeast using fluoride selection

    Depositor

    Julien Berro

    Article

    Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJAT77

    Plasmid

    #204295

    Purpose

    pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX854

    Plasmid

    #62886

    Purpose

    C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa573-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS SV40, and NLS nucleoplasmin

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRN1120

    Plasmid

    #101750

    Purpose

    Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. LRG2.1-CyOFP1

    Plasmid

    #124771

    Purpose

    3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL3287

    Plasmid

    #139750

    Purpose

    Base Cas12a CRISPRi plasmid

    Depositor

    Himadri Pakrasi

    Article

    Knoot et al ACS Synth Biol. 2020 Jan 17;9(1):132-143

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJW1219

    Plasmid

    #61250

    Purpose

    CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E)
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6 promoter

    Available Since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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