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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,310 results for: eng

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Showing: 4041 - 4060 of 14310 results

  1. ePE6d

    Plasmid
    #229029
    Purpose
    Expresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in rice
    Depositor
    Li Juan
    Publication
    Xu et al Plant Biotechnol J. 2024 Sep 27. doi: 10
    Insert
    Cas9
    Use
    CRISPR
    Available since
    Feb. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    229029-plasmid-map-sequence-id-462729

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  5. pcDNA3-sPDGFRalpha-Fc

    Plasmid
    #136438
    Purpose
    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    PDGFRalpha (PDGFRA Human)
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…
    Expression
    Mammalian
    Mutation
    Extracellular domain of PDGFR-alpha (a.a. 1-524)
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136438-plasmid-map-sequence-id-271697

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  7. pHBintN

    Plasmid
    #48135
    Purpose
    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker neomycine for B. megaterium, integration vector
    Depositor
    Dieter Jahn
    Publication
    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48135-plasmid-map-sequence-id-69940

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  11. pCACTUS

    Plasmid
    #167559
    Purpose
    Suicide vector with the sacB gene (as a contraselective marker), CmlR gene, and temperature sensitive ori, used to perform allelic exchange mutagenesis.
    Depositor
    Renato Morona
    Publication
    Morona et al J Bacteriol. 1995 Feb;177(4):1059-68. do
    Type
    Empty backbone
    Use
    Suicide cloning vector, temperature sensitive.
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    167559-plasmid-map-sequence-id-332373

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  16. pGGY003

    Plasmid
    #48867
    Purpose
    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.
    Depositor
    Jan Lohmann
    Publication
    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:
    Type
    Empty backbone
    Use
    Golden gate compatible plant transformation vector
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    48867-plasmid-map-sequence-id-71302

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  19. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

    Plasmid
    #129026
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129026-plasmid-map-sequence-id-256128

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Showing: 4041 - 4060 of 14310 results