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POT5-TEF2p-ycrtYB-ADH1t

Plasmid

#65359

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtYB-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT2-TEF2p-ycrtE-ADH1t

Plasmid

#65353

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtE-ADH1t

Use

Unspecified

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pET-s34T

Plasmid

#28256

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

single chain H3-H4 with Thrombin site

Tags

His

Expression

Bacterial

Mutation

A thrombin cutting site between H3 and H4

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pJC45BS104 Y819W

Plasmid

#1307

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y819W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 819 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pME-Gal80

Plasmid

#51620

Purpose

vertebrate expression of full-length Gal80

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80 (GAL80 Budding Yeast)

Use

Vertebrate expression

Available Since

March 27, 2014

Availability

Academic Institutions and Nonprofits only

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pILGFP4I

Plasmid

#104504

Purpose

Used to evaluate the expression output of Saccharomyces kudriavzevii GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SkGAL1 promoter-yEGFP

Expression

Yeast

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4Q

Plasmid

#104512

Purpose

Used to evaluate the expression output of Saccharomyces kudriavzevii GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SkGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS16-iGFPx6

Plasmid

#105275

Purpose

Plasmid for pop-in/pop-out replacement of Vps16 with Vps16-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps16 portion (VPS16 Budding Yeast)

Tags

iGFPx6

Expression

Yeast

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-Kan

Plasmid

#188923

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Kan

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4L

Plasmid

#104507

Purpose

Used to evaluate the expression output of Saccharomyces mikatae GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SmGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Nov. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pTH646-2µ-RLuc

Plasmid

#40600

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

Renilla Luciferase

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pILGFP4H

Plasmid

#104503

Purpose

Used to evaluate the expression output of Saccharomyces arboricola GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SaGAL1 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4J

Plasmid

#104505

Purpose

Used to evaluate the expression output of Saccharomyces mikatae GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SmGAL1 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4N

Plasmid

#104509

Purpose

Used to evaluate the expression output of Saccharomyces bayanus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SbGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4R

Plasmid

#104513

Purpose

Used to evaluate the expression output of Saccharomyces uvarum GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SuGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLNL1133

Plasmid

#160864

Purpose

PJ23110-RBS-ScEEB1-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Medium-chain fatty acid ethyl ester synthase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Promoter

PJ23110

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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POT4-TDH3p-ycrtI-ADH1t

Plasmid

#65355

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TDH3p-ycrtI-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pDS255

Plasmid

#34970

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

T406A, T407A

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCSJ125

Plasmid

#104344

Purpose

Express yeast MDH2-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

Depositor

Alexander Varshavsky

Article

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

MDH2

Tags

3xFLAG

Expression

Yeast

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRS416 Hsh155 WT

Plasmid

#87219

Purpose

Plasmid used to maintain viability after deletion of genomic copy of HSH155.

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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