167,564 results

1. T7opt in pCAGGS

   Plasmid

   #65974

   Purpose

   Human codon-optimized T7 polymerase

   Depositor

   Benhur Lee

   Article

   Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

   Insert

   T7-opt
   

   Expression

   Mammalian

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. His6-TEV-CRBN-midi

   Plasmid

   #215330

   Purpose

   CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

   Depositor

   Alessio Ciulli

   Article

   Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

   Insert

   CRBN (CRBN Human)
   

   Tags

   6xHis and TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 41-187 followed by a Gly-Ser…

   Promoter

   lacI

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-AAV-syn-jGCaMP8s-WPRE (AAV1)

   Viral Prep

   #162374-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP8s-WPRE (#162374). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8s-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   Syn

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

4. AAV-pgk-Cre (AAV Retrograde)

   Viral Prep

   #24593-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from AAV-pgk-Cre (#24593). In addition to the viral particles, you will also receive purified AAV-pgk-Cre plasmid DNA. Cre expression under the PGK promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.

   Depositor

   Patrick Aebischer

   Promoter

   pgk

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pCDNA3.1(+)-CMV-bArrestin2-TEV

   Plasmid

   #107245

   Purpose

   Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ARRB2
   

   Tags

   TEV(NIA), tobacco etch virus NIA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCXLE-hSK

   Plasmid

   #27078

   Purpose

   Integration-free (episomal) expression of human SOX2 and KLF4

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   SOX2, KLF4 (KLF4, SOX2 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-GRAB_ACh3.0

   Plasmid

   #121922

   Purpose

   express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

   Depositor

   Yulong Li

   Article

   Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

   Has Service

   AAV9

   Insert

   GPCR activation based ACh sensor GRAB-ACh3.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENN.AAV.CMVs.Pl.Cre.rBG (AAV1)

   Viral Prep

   #105537-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   CMV

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pAAV.GfaABC1D.PI.Lck-GFP.SV40 (AAV5)

   Viral Prep

   #105598-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV.GfaABC1D.PI.Lck-GFP.SV40 (#105598). In addition to the viral particles, you will also receive purified pAAV.GfaABC1D.PI.Lck-GFP.SV40 plasmid DNA. GfaABC1D-driven GFP expression control. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Baljit Khakh

   Article

   Shigetomi et al J Gen Physiol. 2013 Apr 15.

   Promoter

   GfaABC1D

   Tags

   GFP

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

10. AAV-GfaABC1D-MCS--4x6T-WPRE

    Plasmid

    #196417

    Purpose

    Empty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Type

    Empty backbone

    Use

    AAV; Astrocyte-selective

    Expression

    Mammalian

    Promoter

    GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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