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Plasmid#11651PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generationDepositorHas ServiceCloning Grade DNAInserthUbiquitin C, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry
Plasmid#182390PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusionsDepositorInsertsVNp-mNeongreen-His
OmpA-mCherry
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lyn11-CIBN-GFP
Plasmid#79572Purposeexpression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFPDepositorInsertLyn11
UseTagsCIBN (CRY2-binding domain) and EGFPExpressionMammalianMutationG8R (please see depositor comments below)PromoterCMVAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVS2
Plasmid#33144DepositorInsertsLacO (256 copies)
GAL-GFP-kd200-GALpA reporter
UseTagsExpressionYeastMutationGFP is mutated such that amino acids 64-73 (FSYGV…Promoterbidirectional GAL1-GAL10 promoterAvailable sinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
Pink Flamindo
Plasmid#102356Purposebiosensor for cAMP in mammalian cellsDepositorInsertPink Flamindo
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-U6-hSyn::mCherry.3xFLAG-WPRE
Plasmid#120392PurposepAAV plasmid with BspQI sites for insertion of target specific shRNAs in frame of the murine U6 promoter and co expressing mCherry.3XFLAG under the hSyn promoterDepositorTypeEmpty backboneUseAAVTagsmCherry.3XFLAGExpressionMutationPromoterU6Available sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-SNAP-hCB1_IRES_EGFP
Plasmid#223505PurposeExpression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection markerDepositorInsertSNAP-tag-CNR1 and EGFP (CNR1 Human, Synthetic)
UseTagsSNAP-tagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-AbKRS-chIPYE
Plasmid#140020PurposePlasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integrationDepositorInsertAbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
UseTagsFLAGExpressionMammalianMutation1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…PromoterEF1Available sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -