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pLOX-CWBmi1

Plasmid

#12240

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

Bmi1 (Bmi1 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

CMV promoter upstream of mBmi-1. LoxP in 3' …

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52379

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4

Expression

Mammalian

Promoter

OCT4

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-hSOX2-17

Plasmid

#193350

Purpose

Retroviral vector expressing human SOX2-17 chimeric transcription factor (super-SOX)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2-17 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

SOX2-17 is engineered highly cooperative chimeric…

Promoter

5'LTR

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMK290 (mAID-mClover-Hygro)

Plasmid

#72828

Purpose

C-terminal tagging with mAID-mClover using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mClover-Hygro

Use

CRISPR

Tags

mAID-mClover

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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GSK3 alpha pMT2

Plasmid

#15896

Depositor

Jim Woodgett

Article

Woodgett et al EMBO J. 1990 Aug . 9(8):2431-8.

Insert

GSK3 alpha (GSK3A Human)

Expression

Mammalian

Available Since

Nov. 1, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pX330-sgAAVS1

Plasmid

#85802

Purpose

Cas9/CRISPR vector for cut at human AAVS1 gene intron1

Depositor

Roland Friedel

Article

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Insert

PPP1R12C (PPP1R12C Human)

Expression

Mammalian

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-I-SceI

Plasmid

#60960

Purpose

Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

I-SceI restriction enzyme

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-C6F/TdTomato

Plasmid

#140826

Purpose

OCT4, SOX2, KLF4, C-MYC, KLF2, NANOG (C6F) and tdTomato under the control of TRE (Tet-ON)

Depositor

Hideyuki Okano

Article

Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

Inserts

OCT4 (POU5F1 Human)
SOX2 (SOX2 Human)
KLF4 (KLF4 Human)
C-MYC (MYC Human)
KLF2 (KLF2 Human)
NANOG (NANOG Human)
tdTomato

Expression

Mammalian

Mutation

L104P- please see depositor comments

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pOmnibac_ZZ_linker_K963_GFP_SNAP

Plasmid

#159727

Purpose

Expresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tag

Depositor

Ahmet Yildiz

Article

Ferro et al Science. 2022 Jan 21;375(6578):326-331.

Insert

KIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)

Tags

GFP and SNAP tags and ZZ affinity tag

Expression

Insect

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAT9651-BEAR-GFP

Plasmid

#162989

Purpose

BEAR target plasmid with split EGFP and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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