We narrowed to 33,653 results for: IND

1. rtTA-sg-mGreenLantern/EGFP

   Plasmid

   #188482

   Purpose

   gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   mGreenLantern/EGFP sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. ppih.001.177.W133A

   Plasmid

   #137655

   Purpose

   Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

   Depositor

   Tara Davis, Melissa Jurica

   Article

   Rajiv et al Biochem J. 2017 Oct 25;474(21):3689-3704

   Insert

   ppih (PPIH Human)
   

   Use

   Tags

   GST-(His)6-thrombin

   Expression

   Bacterial

   Mutation

   001-177-W133A

   Promoter

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT2h70CRET(#325)

   Plasmid

   #184060

   Purpose

   cyclofen-inducible CRE activation in zebrafish transient transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

   Insert

   CRE-ERT2
   

   Use

   Zebrafish transient transgenesis

   Tags

   Expression

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Hsp70-4

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJAK175

   Plasmid

   #178601

   Purpose

   pAP259 derived plasmid encoding a xylose inducible BitlucOpt

   Depositor

   Robert Fagan

   Article

   Phothichaisri et al Microbiol Spectr. 2022 Apr 4:e0236121. d

   Insert

   Bitlucopt
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pxyl

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459-sg-CMV152

   Plasmid

   #179916

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-sg-tdTomato166/892

   Plasmid

   #179919

   Purpose

   Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   tdTomato sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459-sg-CMV51

   Plasmid

   #179915

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX459-sg-CMV162

   Plasmid

   #179917

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-sg-mGreenLantern332

   Plasmid

   #179914

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   mGreenLantern sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLN492 (FuGW-G8p-antiPD1(HC)-1Pe)

    Plasmid

    #105194

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab heavy chain transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-antiPD1(HC)-1Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLN493 (FuGW-G8p-antiPD1(LC)-1Pe)

    Plasmid

    #105195

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab light chain transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-antiPD1(LC)-1Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. NmMetQ

    Plasmid

    #172111

    Purpose

    Expression of Methionine Binding Protein (MetQ) with signal sequence

    Depositor

    Douglas Rees

    Article

    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

    Insert

    Methionine Binding Protein
    

    Use

    Tags

    10x Histidine Tag and N.meningitidis signal seque…

    Expression

    Bacterial

    Mutation

    Changed Proline 22 to Alanine

    Promoter

    T7 Promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLIC-His-GBAi

    Plasmid

    #171755

    Purpose

    For the expression of His-GBAi in bacteria

    Depositor

    Mikel Garcia-Marcos

    Article

    Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

    Insert

    GBAi
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. Tol2-GgVCL/*TBM

    Plasmid

    #162790

    Purpose

    MUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cells

    Depositor

    Laura Lafon-Hughes

    Article

    et al PeerJ

    Insert

    Mutated Gallus gallus vinculin (G454V) (VCL Chicken)
    

    Use

    Tags

    IRES-GFP

    Expression

    Bacterial and Mammalian

    Mutation

    changed Gly 454 to Val

    Promoter

    beta actin

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS406 ATG1-ATG7-3'UTR

    Plasmid

    #166847

    Purpose

    Expresses Atg1 with ATG7 3'UTR in yeasts.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

    Insert

    ATG1 (ATG1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ATG1 Promoter

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS405 VAC8-G2A,C4A,C5A,C7A-RFP

    Plasmid

    #166842

    Purpose

    Expresses a myristoylation and palmitoylation mutant Vac8-RFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7Ala

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8 Budding Yeast)
    

    Use

    Tags

    RFP

    Expression

    Yeast

    Mutation

    G2A, C4A, C5A, C7A

    Promoter

    VAC8 promoter

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS405 VAC8-G2A,C4A,C5A,C7A-GFP

    Plasmid

    #166839

    Purpose

    Expresses a myristoylation and palmitoylation mutant Vac8-GFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7Ala

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    G2A, C4A, C5A, C7A

    Promoter

    VAC8 promoter

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS405 VAC8-C4A,C5A,C7A-GFP

    Plasmid

    #166838

    Purpose

    Expresses a palmitoylation mutant Vac8-GFP in yeasts, mutations are Cys4Ala, Cys5Ala, Cys7Ala

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    C4A, C5A, C7A

    Promoter

    VAC8 promoter

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-Gal4-RNF169UBD

    Plasmid

    #119013

    Purpose

    Mammalian expression of fusion protein of yeast Gal4 with human RNF169 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    Gal4 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA4/TO-mu24 1-181-2xStrep

    Plasmid

    #138444

    Purpose

    Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    mu24
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-181

    Promoter

    CMV

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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