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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,639 results for: pcDNA 3.1

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Showing: 4061 - 4080 of 7639 results

  11. pcDNA3.2-Cx43-NoStart

    Plasmid
    #49853
    Purpose
    Encodes human connexin 43 with the start methionine codon mutated to isoleucine and with wobble mutations conferring resistance against an siRNA
    Depositor
    Robin Shaw
    Article
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Insert
    Connexin 43 (GJA1 Human)
    Expression
    Mammalian
    Mutation
    Start codon mutated to from methionine to isoleuc…
    Promoter
    CMV
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pcDNA3.BRWD3-V5H

    Plasmid
    #48624
    Purpose
    Expresses Drosophila BRWD3 (with V5 and His tags at the C-terminus) in mammalian cells
    Depositor
    Aziz Sancar
    Article
    Ozturk et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    BRWD3 (BRWD3 Fly)
    Tags
    V5 and His tags
    Expression
    Mammalian
    Mutation
    no mutations, stop was removed to fuse V5His at C…
    Promoter
    CMV
    Available Since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. pcDNA3.2-GJA1-20K-V5

    Plasmid
    #49859
    Purpose
    Encodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tag
    Depositor
    Robin Shaw
    Article
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Insert
    GJA1-20k (GJA1 Human)
    Tags
    V5
    Expression
    Mammalian
    Mutation
    GJA1-20k fragment corresponds to residues 213-382…
    Promoter
    CMV
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-99784

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  18. pcDNA-zeo-hFJX1-myc

    Plasmid
    #46930
    Purpose
    expression of human FJX1-myc in mammalian cells
    Depositor
    R.D. Beauchamp, Natasha Deane
    Insert
    FJX1 (FJX1 Human)
    Tags
    myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 4061 - 4080 of 7639 results