We narrowed to 4,482 results for: ARA-2

1. pEGFP-C1 Slp2-a E11A/R32A

   Plasmid

   #40055

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   Slp2-a E11A/R32A (Sytl2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Glutamate 11 to Alanine, Arginine 32 to Alanine

   Promoter

   CMV promoter

   Available since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pEGFP-N1 Btsz2

   Plasmid

   #40068

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   Btsz2 (btsz Fly)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Oct. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

3. MKC131_CCR5(SFFV-synEPOR-2A-YFP)

   Plasmid

   #232412

   Purpose

   AAV production plasmid for SFFV(synEPOR) vector from Figs. 2-3 that mediates HDR at CCR5 locus using CCR5 gRNA. YFP is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.

   Depositor

   Kyle Cromer

   Article

   Shah et al bioRxiv 2024.04.12.589260

   Insert

   Truncated Erythropoietin Receptor (EPOR Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T1_GST-GBRPL1

   Plasmid

   #223729

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   GABARAPL1 (GABARAPL1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 2-116 only, with Glycine exposed

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOGS539_GLuc

   Plasmid

   #226718

   Purpose

   Plasmid enabling yeast-mediated expression and secretion of Gaussia luciferase (GLuc)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Gaussia Luciferase
   

   Use

   Tags

   HA tag and Mating factor alpha secretion signal

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDest17_hnRNPA1_FtoW

   Plasmid

   #224254

   Purpose

   Bacterial expression of N-terminally 6His-tagged hnRNPA1_FtoW

   Depositor

   Tanja Mittag

   Article

   Alshareedah et al Nat Phys. 2024 Sep;20(9):1482-1491. doi:

   Insert

   hnRNPA1_FtoW (HNRNPA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F17W, F25W, F31W, F37W, F43W, F62W, F69W, F78W, F…

   Promoter

   T7

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGhMeCP2 (pc3972)

   Plasmid

   #212033

   Purpose

   Expresses human MeCP2 tagged N-terminal to Halo-tag

   Depositor

   Cristina Cardoso

   Article

   Zhang et al Nucleus. 2022 Dec;13(1):1-34. doi: 10.10

   Insert

   MeCP2 (MECP2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmxs_COX4

   Plasmid

   #177979

   Purpose

   Mammalian expression of COX4 cDNA

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   COX4 (COX4I1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Silent point mutations to COX4 sgRNA target

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1-TRC.mKO2_shHRASLS.1

   Plasmid

   #110323

   Purpose

   TRCN0000378630 (Target GATAAGTACCGTTGAGTTTG), silence human HRASLS gene and express monomeric Kusabira-Orange2

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   HRASLS (PLAAT1 Human, Homo sapiens)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion (NT96)

    Plasmid

    #50914

    Purpose

    Expresses human NKCC1 with truncation of N-terminus and mutations at P676C I730C. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    P676C I730C N-term deletion of aa13-222 in hNKCC1…

    Promoter

    CMV

    Available since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 Flag YFP hNKCC1 C723S C724V A735C (NT549)

    Plasmid

    #50910

    Purpose

    Expresses human NKCC1 C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S C724V A735C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 Flag YFP hNKCC1 A675C C723S C724V A735C (NT847)

    Plasmid

    #50865

    Purpose

    Expresses human NKCC1 A675C C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    A675C C723S C724V A735C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1 Flag YFP hNKCC1 C723S C724V V729C (NT543)

    Plasmid

    #50907

    Purpose

    Expresses human NKCC1 C723S C724V V729C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S C724V V729C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 Flag YFP hNKCC1 C723S C724V W733C (NT547)

    Plasmid

    #50909

    Purpose

    Expresses human NKCC1 C723S C724V W733C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S C724V W733C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1 Flag YFP hNKCC1 C723S C724V F728C (NT542)

    Plasmid

    #50906

    Purpose

    Expresses human NKCC1 C723S C724V F728C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S C724V F728C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 Flag YFP hNKCC1 C723S C724V M727C (NT540)

    Plasmid

    #50905

    Purpose

    Expresses human NKCC1 C723S C724V M727C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S C724V M727C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXS-IRES-BLAST flag-CAD

    Plasmid

    #169818

    Purpose

    Expresses C-terminal flag-tagged CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCCC -> AGTC silent mutations at nt527-530 eli…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AICSDP-23:TJP1-mEGFP

    Plasmid

    #87429

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pScalps_Puro_mTet2 catalytic domain HxD

    Plasmid

    #79611

    Purpose

    Expression of catalytically inactive mouse Tet2

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Tet2 (Tet2 Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mutation

    Mutant Tet2 with H1302Y, D1304A substitutions in …

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pVD1 tRNA-gRNA position E2 (GB2239)

    Plasmid

    #160561

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNA

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    tRNA-gRNA position E2 (Multiplexing Edit)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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