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pKmK.P10

Plasmid

#125043

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

GDH2 promoter

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.P11

Plasmid

#125044

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

HHF1 promoter

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGGA000

Plasmid

#48856

Purpose

Empty entry vector for creating GreenGate promoter modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-Myc-MBP-HA

Plasmid

#222711

Purpose

Expression Myc-tagged F-box protein and MBP-HA-tagged substrate in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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PLlacO-1-mfLon

Plasmid

#45152

Depositor

Michel Maharbiz

Article

Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Insert

Lon

Use

Synthetic Biology

Tags

ssRA degradation (LVA)

Expression

Bacterial

Promoter

PLlacO-1

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pdestDD04Neo

Plasmid

#26386

Depositor

Denis Dupuy

Article

Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

Type

Empty backbone

Expression

Bacterial and Worm

Available Since

Oct. 29, 2010

Availability

Academic Institutions and Nonprofits only

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Ubi:3xHA-ccdb + RUBY

Plasmid

#233307

Purpose

GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of 3xHA tag paired with ccdb selection toxin and RUBY marker

Depositor

Thomas Baum

Insert

Ubi:3xHA-ccdb + RUBY

Use

Gateway destination

Tags

3xHA

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-knt-P-R*

Plasmid

#189852

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P-loxP-TT-loxP-R*-knt

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P (T7 phage gene 10)

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGGC000

Plasmid

#48858

Purpose

Empty entry vector for creating GreenGate coding sequence modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pACRISPR-CM

Plasmid

#203588

Purpose

A sgRNA expression plasmid for genome editing in Burkholderia

Depositor

Leonilde Moreira

Article

Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

Type

Empty backbone

Expression

Bacterial

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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lvl0 eCFP CDS

Plasmid

#153397

Purpose

Cyan fluorescent protein coding sequence

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

eCFP CDS

Expression

Plant

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 lexA-35S

Plasmid

#153407

Purpose

Estradiol system inducible promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

lexA-35S promoter

Expression

Plant

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJMP2820

Plasmid

#160669

Purpose

Mobile CRISPRi sfGFP 'test', empty sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

empty sgRNA (BsaI)

Expression

Bacterial

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-knt-P*-R

Plasmid

#189850

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P*-loxP-TT-loxP-R-knt

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P* (modified T7 phage gene 10)

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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lvl0 mVenus CDS

Plasmid

#153403

Purpose

Yellow fluorescent protein coding sequence

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

mVenus CDS

Expression

Plant

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBLO 9.3_bCas9-CP230

Plasmid

#121497

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMYT013

Plasmid

#180666

Purpose

Encodes pTet5 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

pTet5

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pVTU-Dest

Plasmid

#105088

Purpose

Yeast expression vector for Gateway cloning and SUS bridge assays

Depositor

Christopher Grefen

Article

Grefen et al Methods Mol Biol. 2009;479:217-33. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pGCS-C3m(3xFLAG)

Plasmid

#85726

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xFLAG

Available Since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pZa-tetO-csgB-CmR

Plasmid

#89580

Purpose

Express csgB curli protein

Depositor

Urartu Seker

Article

Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

Insert

csgB

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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