We narrowed to 17,999 results for: puro
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pEN243-EN1953
Plasmid#224546PurposeCas9-2A-puro and sgRNA targeting the first coding ATG of mouse NipblDepositorInsertCas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl (Nipbl Mouse)
ExpressionMammalianAvailable SinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
CROP-seq-mTurquoise2-v2-no-scaffold
Plasmid#228522PurposeCROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection markerDepositorInsertmTurquoise2
UseLentiviralAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROP-seq-copGFP-v2-no-scaffold
Plasmid#228520PurposeCROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection markerDepositorTypeEmpty backboneUseLentiviralAvailable SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX459v3-eSpCas9(1.1)
Plasmid#178800PurposeHigh-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available SinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX459v3-SpCas9-HF1
Plasmid#178801PurposeHigh-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available SinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PIG-N3FLAG-TAF12(50-130)
Plasmid#105595Purposeretrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistanceDepositorInsertTAF12 (AA 50-130)- histone fold domain-HFD (Taf12 Mouse)
UseRetroviralTags3*FLAGExpressionMammalianMutationtruncation fragments containing aa (50-130), and …PromoterMSCV-LTRAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Barcoded self-reporting transposon calling card collection
Plasmid Kit#1000000213Purpose'Calling Cards' technology using self-reporting transposons (SRTs) enables the identification of DNA-protein interactions through RNA sequencing.DepositorAvailable SinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
CRISPaint Gene Tagging Kit
Plasmid Kit#1000000086PurposeModular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tagsDepositorAvailable SinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1
Plasmid#41393Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vector, doxycycli…ExpressionMammalianPromoterTRE promoter, Tet ONAvailable SinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLIX_403
Plasmid#41395PurposeInducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTADepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vector, doxycycli…TagsV5ExpressionMammalianPromoterTRE promoter, Tet ONAvailable SinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Neo
Plasmid#127762PurposeUsed for expression of sgRNA in mammalian cellsDepositorTypeEmpty backboneExpressionMammalianAvailable SinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Hygro
Plasmid#127763PurposeUsed for expression of sgRNA in mammalian cellsDepositorTypeEmpty backboneExpressionMammalianAvailable SinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmx1-V5
Plasmid#175138PurposeLentiviral expression of V5-tagged mouse Tmx1DepositorAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Nesprin-3
Plasmid#175136PurposeLentiviral expression of V5-tagged mouse Syne3DepositorAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
FU_tetO_dCas9-VP192-T2A-EGFP
Plasmid#121173PurposeLentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.DepositorInsertdCas9-VP192-T2A-EGFP
UseCRISPR and Lentiviral; Inducible by doxycyclineTagsT2A-EGFP-ires-puroExpressionMammalianAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB700-mouse-TTN-Cerulean
Plasmid#128325PurposeSP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)DepositorInsertgRNA against mouse TTN for activation
ExpressionMammalianAvailable SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
katushka-FMDV-P2A-pTRIP
Plasmid#163522PurposeUsed to construct lentivirus to express katushka in mammalian cells with option for a second insertDepositorInsertstopless Katushka-P2A
UseLentiviralExpressionBacterial and MammalianAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem9b-V5
Plasmid#175152PurposeLentiviral expression of mouse Tmem9b-V5DepositorAvailable SinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only
