Addgene

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mutpRL-TK 1xUTR

Plasmid

#40756

Purpose

To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Expression

Mutation

mutated from TAG to CTC at nt 387–389 of the Reni…

Promoter

TK

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
CS2-Myc-dLIS1

Plasmid

#47047

Purpose

Expression of tagged Drosophila LIS1

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Lis1 (Lis-1 Fly)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only
CS2-CHY-dLIS1

Plasmid

#47045

Purpose

Expression of fluorescent Drosophila LIS1

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Lis1 (Lis-1 Fly)

Use

Tags

CHY

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only
pDM10

Plasmid

#41759

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc Fly, E. coli)

Use

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C45S C338S A378C C451S

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pDM8

Plasmid

#41758

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc Fly, E. coli)

Use

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C45S C338S S364C C451S

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pOU1

Plasmid

#41753

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc Fly, E. coli)

Use

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C338S C451S

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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ND4.2-Right DdCBE-G1397-C- T1413I-GFP

Plasmid

#179686

Purpose

Expression of base editor in mammalian cells and for cell sorting

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

T1413I

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

Plasmid

#114595

Purpose

Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

Depositor

Brian Chow

Article

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pBK-CMV-hNHERF2

Plasmid

#47801

Purpose

Expresses human NHERF2 in mammalian cells

Depositor

Randy Hall

Article

Lau et al Biochemistry. 2001 Jul 24;40(29):8572-80

Insert

NHERF2 (NHERF2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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MAC-NRP1

Plasmid

#158384

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

NRP1 (NRP1 Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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prDNA spacer-LacOx50-20xMS2-Hyg

Plasmid

#210219

Purpose

Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMX-mTAF6

Plasmid

#44337

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF6

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR221_HUWE1_isoform1

Plasmid

#190464

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMD2-VSVGmut

Plasmid

#182229

Purpose

Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

VSVGmut

Use

Tags

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR223-BALF4

Plasmid

#195835

Purpose

Gateway Entry Clone of Epstein-Barr virus BALF4

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BALF4 (BALF4 Epstein-Barr virus)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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anti-FLAG M2 light chain

Plasmid

#175358

Purpose

Mammalian expression vector for over-expression of anti-FLAG M2 light chain

Depositor

Joost Snijder

Article

Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

Insert

anti-FLAG M2 light chain

Use

Tags

N-terminal secretion signal peptide (MEAPAQLLFLLL…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV FLEX Synaptophysin GFP

Plasmid

#137188

Purpose

Cre-dependent expression of synaptophysin-GFP

Depositor

Matthew Nolan

Article

Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

Insert

Synaptophysin GFP (Syp Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Lambda Phosphatase

Plasmid

#79748

Purpose

Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

Depositor

Markus Seeliger, Nicholas Levinson, John Chodera

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

Lambda Phosphatase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pFloat.cspA_v1

Plasmid

#133071

Purpose

Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.

Depositor

David Sabatini, Kacper Rogala

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Entry vector

Tags

3C-His6 and His6-3C

Expression

Bacterial

Mutation

Promoter

cspA

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pME minprom KalTa4

Plasmid

#195964

Purpose

Gateway pME Gal4 variant

Depositor

Alexa Burger, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mouse beta-globin minimal promoter KalTA4 (Gal4)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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