We narrowed to 24,896 results for: Nes;

1. hCMV-IE1:Lucia:bgH

   Plasmid

   #135293

   Purpose

   Transcriptional unit for constitutive expression of Lucia luciferase in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:Lucia:bGH
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSU314

   Plasmid

   #169696

   Purpose

   Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.

   Depositor

   Nara Figueroa

   Article

   Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

   Insert

   1xHA epitope tag adjacent to FRT-flanked cat (CamR) gene
   

   Use

   Tags

   1xHA tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOpLexA::mini35s (GB0733)

   Plasmid

   #68180

   Purpose

   Provides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   4xOpLexA:mini35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXylA-RgTALsyn

   Plasmid

   #100948

   Purpose

   Expression of Tyrosine Ammonia Lyase from constitutive XylA promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Insert

   RgTALsyn
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   XylA (Constitutive)

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDGB1omega2_SF (GB0466)

   Plasmid

   #68243

   Purpose

   Twister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vector

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Solanum lycopersicum intergenic region
   

   Use

   Synthetic Biology; Unspecified

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGB1alpha2_SF (GB0107)

   Plasmid

   #68233

   Purpose

   Twister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vector

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Solanum lycopersicum intergenic region
   

   Use

   Synthetic Biology; Unspecified

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSU312

   Plasmid

   #169694

   Purpose

   Template plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

   Depositor

   Nara Figueroa

   Article

   Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

   Insert

   1xFLAG epitope tag adjacent to FRT-flanked aph (KanR) gene
   

   Use

   Tags

   1xFLAG tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSU313

   Plasmid

   #169695

   Purpose

   Template plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

   Depositor

   Nara Figueroa

   Article

   Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

   Insert

   1xFLAG epitope tag adjacent to FRT-flanked cat (CamR) gene
   

   Use

   Tags

   1xFLAG tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-HA-mIFITM1-C49,50,83,103A

   Plasmid

   #58421

   Purpose

   Expresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in mammalian cells (cysteine-less mutant that is not palmitoylated).

   Depositor

   Jacob Yount

   Article

   Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

   Insert

   IFITM1 (Ifitm1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Cysteines 49, 50, 83, and 103 to Alanine

   Promoter

   CMV IE

   Available since

   Sept. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

10. AAV-CAG-DIO-mAPX

    Plasmid

    #79908

    Purpose

    Adeno-associated virus plasmid, Cre-dependent FLEX switch, mAPX

    Depositor

    Joshua Sanes

    Article

    Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

    Insert

    mAPX
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTHsp18.2 (GB0035)

    Plasmid

    #68186

    Purpose

    Provides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtHsp18.2 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)

    Plasmid

    #178996

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)

    Plasmid

    #178995

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nano-lantern-H2B/pcDNA3

    Plasmid

    #51971

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to Histone H2B

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    human H2B

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pOpLacI:mini35ss (GB0534)

    Plasmid

    #68179

    Purpose

    Provides the 6xLacI Operon fused to CaMV 35S minimal promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    6xOpLacI:mini35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. A337C

    Plasmid

    #24432

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C

    Promoter

    Available since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

17. Venus-Cdc20 R132A (1768)

    Plasmid

    #26059

    Depositor

    Jonathon Pines

    Article

    Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

    Insert

    Cdc20 (CDC20 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Mutation in the Mad2 binding site, R132A

    Promoter

    Available since

    Aug. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

18. p6xAP-1_RE

    Plasmid

    #118054

    Purpose

    GBPart - Operator/DNA Response element - 6 copies of the AP-1 binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    6 copies of the AP-1 binding motif
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. YFP(1-158) in pcDNAI/Amp

    Plasmid

    #60888

    Purpose

    This vector attaches YFP(1-158) to the N-terminus of a protein.

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

    Insert

    YFP(1-158)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Met was substituted for Gln-69.

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUPD_OsU3 (GB1184)

    Plasmid

    #68209

    Purpose

    Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    OsU3 promoter
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge