We narrowed to 9,954 results for: ada

1. plko_TRE3G_Myc-Sox9HMGonlySTOP_PGKpuroR

   Plasmid

   #203963

   Purpose

   Dox inducible myc tag truncated SOX9 without trans-activating domain (noTA)

   Depositor

   Elaine Fuchs

   Article

   Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

   Insert

   SOX9-noTA (Sox9 Mouse)
   

   Use

   Lentiviral

   Tags

   myc

   Expression

   Mutation

   the TA domain is truncated

   Promoter

   TRE3G

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. plko_TRE3G_nlsMyc-Sox9HMGtruncation_PGKpuroR

   Plasmid

   #203964

   Purpose

   Dox inducible myc tag truncated SOX9 without DNA-binding domain (noHMG)

   Depositor

   Elaine Fuchs

   Article

   Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

   Insert

   SOX9-noHMG (Sox9 Mouse)
   

   Use

   Lentiviral

   Tags

   myc

   Expression

   Mutation

   the HMG domain is truncated

   Promoter

   TRE3G

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3xMS-TR-100-PURO HRD

   Plasmid

   #207582

   Purpose

   homologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
   

   Use

   Tags

   3xMS2

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACEBac1_Strep-Psc-BICD2(711-800)_L786A

   Plasmid

   #228838

   Purpose

   Insect cell expression of the mutant C-terminus of BICD2

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   BICD2 (Bicd2 Mouse)
   

   Use

   Tags

   Strep

   Expression

   Insect

   Mutation

   aa 711-800, with L786A

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACEBac1_Strep-Psc-BICD2(711-800)

   Plasmid

   #228833

   Purpose

   Insect cell expression of CC3 domain of BICD2 (aa 711-800)

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   BICD2 (Bicd2 Mouse)
   

   Use

   Tags

   Strep

   Expression

   Insect

   Mutation

   aa 711-800

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACEBac1_Strep-Psc-BICD2(342-800)

   Plasmid

   #228832

   Purpose

   Insect cell expression of C-terminal portion of BICD2 (aa 342-800)

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   BICD2 (Bicd2 Mouse)
   

   Use

   Tags

   Strep

   Expression

   Insect

   Mutation

   aa 342-800

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACEBac1_Strep-Psc-BICD2(711-800)_L742A

   Plasmid

   #228839

   Purpose

   Insect cell expression of the mutant C-terminus of BICD2

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   BICD2 (Bicd2 Mouse)
   

   Use

   Tags

   Strep

   Expression

   Insect

   Mutation

   aa 711-800, with L742A

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMT-mMyh13CDS

   Plasmid

   #192853

   Purpose

   Plasmid to produce ISH probe for mMyh13 gene with CDS sequence

   Depositor

   Masafumi Inui

   Article

   Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

   Insert

   myosin heavy chain 13 (Myh13 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEMT-mMyh13UTR

   Plasmid

   #192854

   Purpose

   Plasmid to produce ISH probe for mMyh13 gene with UTR sequence

   Depositor

   Masafumi Inui

   Article

   Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

   Insert

   myosin heavy chain 13 (Myh13 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAM59

    Plasmid

    #227655

    Purpose

    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, Δ78–81)

    Depositor

    Andreas Martin

    Article

    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    H6-PreScission and PreScission-Strep

    Expression

    Bacterial

    Mutation

    1-179 and amino acids 2-239, Δ78–81

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM57

    Plasmid

    #227654

    Purpose

    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, V80A)

    Depositor

    Andreas Martin

    Article

    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    H6-PreScission and PreScission-Strep

    Expression

    Bacterial

    Mutation

    1-179 and amino acids 2-239, V80A

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAM54

    Plasmid

    #227653

    Purpose

    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, L132A)

    Depositor

    Andreas Martin

    Article

    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    H6-PreScission and PreScission-Strep

    Expression

    Bacterial

    Mutation

    1-179 and amino acids 2-239, L132A

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHM148

    Plasmid

    #225532

    Purpose

    Encodes constitutive rtTA, tetOn-Pcdh19-GFP, and constitutive mCherry

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Inserts

    rtTA
    Pcdh19 (Pcdh19 Mouse)
    mCherry
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-PCNT

    Plasmid

    #227284

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of PCNT for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of PCNT (PCNT Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-AAVS1-PGK-Zeo

    Plasmid

    #227271

    Purpose

    Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX458-PLK4

    Plasmid

    #227310

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of PLK4 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PLK4 (PLK4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. WDV

    Plasmid

    #226983

    Purpose

    Expresses the HUH endonuclease WDV, used as a no ligand control for DNA tension sensor experiments

    Depositor

    Wendy Gordon

    Article

    Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

    Insert

    WDV (repA )
    

    Use

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDIV272

    Plasmid

    #226183

    Purpose

    Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

    Insert

    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TEF1p (Komagataella phaffii)

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-cT3G-cERP2-SOD1

    Plasmid

    #222568

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of SOD1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-cT3G-cERP2-KDM5B

    Plasmid

    #222555

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of KDM5B

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    KDM5B (KDM5B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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