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pHR_EF1a-->HA-mtLaG2

Plasmid

#162225

Purpose

Lentiviral vector for the expression of HA GFP nanobody Lag2 in mammalian cells

Depositor

Wendell Lim

Publication

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-GFP nanobody LaG2

Use

Lentiviral

Expression

Mammalian

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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p32Lb

Plasmid

#158154

Purpose

Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and FnCpf1, respectively. For plant genome editing.

Depositor

Kabin Xie

Publication

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB6627

Plasmid

#106159

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6682 into Yarrowia lipolytica chromosomal location IntC_2, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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2xNLS-pMJ915v2

Plasmid

#88916

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Publication

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTXG

Plasmid

#52280

Purpose

Bacterial expression of TARGET proteins C-terminally fused to GyrA intein and GST

Depositor

Primo Schaer

Publication

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Type

Empty backbone

Tags

GyrA-intein-GST

Expression

Bacterial

Promoter

T7

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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BBa_J72114-BBa_J72100

Plasmid

#40781

Depositor

Christopher Anderson

Publication

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

lacZ

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pTHSSe_43

Plasmid

#109241

Purpose

pSC101 var1 BbsI cloning vector

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAtCas9_1

Plasmid

#91156

Purpose

Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Expression

Plant

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDIRECT_10A

Plasmid

#91207

Purpose

Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Expression

Plant

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pKMV-VioA

Plasmid

#65347

Purpose

systhesis of vioA gene in the violacein pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioA

Use

Synthetic Biology

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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IFP1.4-C1

Plasmid

#54782

Purpose

Localization: C1 Cloning Vector, Excitation: 684, Emission: 708

Depositor

Michael Davidson, Roger Tsien

Publication

Shu et al Science. 2009 May 8;324(5928):804-7. doi

Type

Empty backbone

Tags

IFP1.4

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGGG002

Plasmid

#48851

Purpose

Provides an H-A adapter as GreenGate module.

Depositor

Jan Lohmann

Publication

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

H-A adapter - short

Use

Golden gate compatible cloning vector

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX458-HypaCas9

Plasmid

#190604

Purpose

PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

Depositor

Yuichiro Miyaoka

Publication

Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pExchange_tdk_BT1311p-tetR-2

Plasmid

#90326

Purpose

Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and 2660694

Depositor

Andrew Goodman

Publication

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

TetR

Expression

Bacterial

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSBDEST.Z

Plasmid

#79470

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Zeocin

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCLX-UBI-GFP

Plasmid

#27245

Depositor

Patrick Salmon

Publication

Salmon et al Methods Mol Biol. 2013;945:417-48. doi:

Insert

GFP

Use

Lentiviral

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHiUGE HA-epitope donor ORF1

Plasmid

#200372

Purpose

Tagging endogenously expressed proteins with HA epitope, C-terminal

Depositor

Scott Soderling

Publication

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

HA epitope

Expression

Mammalian

Mutation

NA

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pP2-P1(S')

Plasmid

#196334

Purpose

Plasmid expressing chimeric P2-P1(S') tail fiber

Depositor

Baojun Wang

Publication

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Insert

chimeric tail fiber P2-P1(S') and chaperone protein U'

Expression

Bacterial

Promoter

P2 late promoter

Available since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pFloxin-IRES-eGFP

Plasmid

#24557

Depositor

Jeremy Reiter

Publication

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Insert

eGFP

Use

Cre/Lox and Mouse Targeting

Available since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET24b-SUMO-BG2.4

Plasmid

#199438

Purpose

expresses BAmGreen2.4 in bacterial cells with a SUMO tag

Depositor

Andrew Woolley

Publication

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

BAMGreen2.4

Tags

his tag and sumo

Expression

Bacterial

Promoter

T7

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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