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pYPQ289-RVR

Plasmid

#138127

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

ErCas12a RVR variant

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

D513R, K519V and N523R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ289-RVR

Plasmid

#138127

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

ErCas12a RVR variant

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

D513R, K519V and N523R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ287-RVR

Plasmid

#138124

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a with N512R, K518V and N522R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

BsCas12a RVR variant

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

N512R, K518V and N522R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ287-RVR

Plasmid

#138124

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a with N512R, K518V and N522R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

BsCas12a RVR variant

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

N512R, K518V and N522R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ285-RVR

Plasmid

#138121

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a with N512R, K518V and N522R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Lb5Cas12a RVR variant

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

N512R, K518V and N522R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ285-RVR

Plasmid

#138121

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a with N512R, K518V and N522R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Lb5Cas12a RVR variant

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

N512R, K518V and N522R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry2_TagBFP

Plasmid

#83031

Purpose

Expresses full length sfCherry2 and TagBFP fusion in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry+full length_TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

3 mutations in sfCherry full length: E118Q, T128I…

Promoter

SFFV

Available Since

Jan. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pXTm-PH domain

Plasmid

#210326

Purpose

Expresses EGFP with PH domain

Depositor

Li Yu

Article

Jiang et al Nat Cell Biol. 2019 Aug;21(8):966-977. d

Insert

PH domain

Use

Fish expression

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET-SisTnpB1–ωRNA

Plasmid

#228365

Purpose

Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

Depositor

Tao Liu

Article

Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

Insert

SisTnpB1

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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PC1-29-9-2_GAL4-VHL

Plasmid

#200434

Purpose

Expresses the GAL4 DNA Binding domain linked to the VHL E3 Ubiquitin Ligase

Depositor

Xue Gao

Article

Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

Insert

GAL4-VHL

Tags

GAL4, VHL

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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PC17-605-9-1_BRD4-BD2-VPR

Plasmid

#200436

Purpose

Expresses the VPR transactivation domain linked to the BRD4(BD2) target protein

Depositor

Xue Gao

Article

Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

Insert

BRD4(BD2)-VPR

Tags

BRD4(BD2), VPR

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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PC15-421-14-1_TRIM24-BD-VPR

Plasmid

#200435

Purpose

Expresses the VPR transactivation domain linked to the TRIM24(BD) target protein

Depositor

Xue Gao

Article

Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

Insert

TRIM24(BD)-VPR

Tags

TRIM24(BD), VPR

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pXTm-zebrafish Tspan4a

Plasmid

#210327

Purpose

Expresses Tetraspanin-4a (TSPAN4a) as migrasome marker

Depositor

Li Yu

Article

Jiang et al Nat Cell Biol. 2019 Aug;21(8):966-977. d

Insert

Tetraspanin 4a (tspan4a Zebrafish)

Use

Fish expression

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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PC7-709-28-1_CRBN-VPR

Plasmid

#200437

Purpose

Expresses the VPR transactivation domain linked to the CRBN E3 Ubiquitin Ligase

Depositor

Xue Gao

Article

Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

Insert

CRBN-VPR

Tags

CRBN, VPR

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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PA-DreN

Plasmid

#169707

Purpose

The optimized DreN246-nMag

Depositor

Dali Li

Article

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Insert

Dre246-nMag

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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PA-DreC

Plasmid

#169705

Purpose

The optimized pMag-Dre247

Depositor

Dali Li

Article

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Insert

pMag-Dre247

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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BPK2014-AaCas12b

Plasmid

#121949

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b

Tags

10xHis tag

Expression

Bacterial

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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BPK2014-AaCas12b

Plasmid

#121949

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b

Tags

10xHis tag

Expression

Bacterial

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b-2AeGFP

Plasmid

#121945

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b

Tags

3xHA tag

Expression

Mammalian

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-AaCas12b-2AeGFP

Plasmid

#121945

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b

Tags

3xHA tag

Expression

Mammalian

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge