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Plasmid#111850PurposeExpresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G12V (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 12 to ValinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBSedit Sham
Plasmid#223200PurposeGroup B Streptococcus Efficient Genome Editing with Modular crRNADepositorInsertN/A
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGBScrispri Sham
Plasmid#223201PurposeGroup B Streptococcus inducible dCas12 CRISPRi with Modular crRNADepositorInsertN/A
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGBScomp High
Plasmid#223202PurposeGroup B Streptococcus High Expression Modular Complementation VectorDepositorInsertN/A
UseComplementation vectorTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGBScomp Low
Plasmid#223203PurposeGroup B Streptococcus Low Expression Modular Complementation VectorDepositorInsertN/A
UseComplementation vectorTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC2483 (pSpy1C)
Plasmid#220248Purposereplicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
766 p3E-2A-nlsmCherrypA
Plasmid#195972PurposeGateway p3E 2A fluorophoreDepositorInsert2A-nlsmCherry (nuclear)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEvoltac-EcW-TGA-h14
Plasmid#218764Purposeencodes EcWRS-h14 and EcW-tRNA-TGADepositorInsertEcWRS-h14
UseTagsExpressionBacterialMutationS8A, V144G, V146CPromotertacIAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
ART
Plasmid#214063PurposeFluorescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SF3B1-K700E-reporter
Plasmid#200318PurposeA fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.DepositorInsertBSD-2A-EGFPi
UseLentiviralTagsExpressionMammalianMutationPromoterEF1-aAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only