We narrowed to 2,346 results for: puc19

1. pDonor-GDH

   Plasmid

   #140629

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   Mini-Tn6677, T7lac-GDH gene cassette
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPN066

   Plasmid

   #91601

   Purpose

   Express sgRNA targeting human DPP4

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   DPP4 (DPP4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. qTAG-C-miRFP670nano3-Blast-H3C2

   Plasmid

   #207784

   Purpose

   Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H3C2 Homology Arms flanking a miRFP670nano3-Blast Cassette (H3C2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. TK-GFP-Nluc-P2A-neo-TK

   Plasmid

   #134896

   Purpose

   Expresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locus

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Insert

   TK-GFP-Nluc-P2A-Neo-TK
   

   Use

   Cryptosporidium expression

   Tags

   Expression

   Mutation

   Promoter

   Cryptosporidium actin and enolase

   Available since

   Nov. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB-XPRESSO-hSpCas9-EGFP

   Plasmid

   #237298

   Purpose

   Sleeping Beauty XPRESSO vector for Cas9-EGFP expression

   Depositor

   Lior Gepstein

   Article

   Wexler et al Stem Cell Reports. 2025 Aug 19:102603. d

   Insert

   spCas9 (cas9 Stretococcus pyogenes)
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-R2Tg_opt-3xFlag

   Plasmid

   #223251

   Purpose

   Mammalian expression of C-terminally Flag-tagged optimized R2Tg protein

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg protein_opt
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AbVec2.0-mIgkc

   Plasmid

   #127157

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant region

   Depositor

   Hedda Wardemann

   Article

   Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

   Insert

   Igkc (Igkc Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AbVec2.0-mIghg2b

   Plasmid

   #127155

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotype

   Depositor

   Hedda Wardemann

   Article

   Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

   Insert

   Ighg2b (Ighg2b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-N-Blast-mNeon-ACTB

   Plasmid

   #207751

   Purpose

   Donor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. IR25a-T2A-QF-AD

    Plasmid

    #162526

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses the activation domain of QF by knocking-in a T2A-QF-AD fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Ir25a-left-HDR-arm; T2A-QF2AD-Zip+-SV40; 3xP3-EYFP-SV40; Ir25a-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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