We narrowed to 9,608 results for: FIE

1. pUb FLAG-Pp2A-29B

   Plasmid

   #195066

   Purpose

   Expresses FLAG-tagged Drosophila Pp2A-29B protein

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   Protein phosphatase 2A at 29B (Pp2A-29B Fly)
   

   Use

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Ubi-p63e

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6P-1-MVP delta607-623aa

   Plasmid

   #204542

   Purpose

   Expresses GST-tagged MVP delta607-623aa in E.coli

   Depositor

   Masaaki Komatsu

   Article

   Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

   Insert

   MVP (MVP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   delta607-623aa

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX459-dMET

   Plasmid

   #176031

   Purpose

   A knock-out vector for dog MET

   Depositor

   Michiyuki Matsuda

   Article

   Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

   Insert

   A gRNA targeting the dog MET gene and the cDNA of Cas9 (MET )
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pv6_MECP2_MBD-R133C

   Plasmid

   #179409

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R133C)

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MECP2_MBD(R133C) (Mecp2 Mouse)
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   MBD R133C

   Promoter

   CAGGS

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pv6_MECP2_MBD-T158M

   Plasmid

   #179410

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(T158M)

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MECP2_MBD(T158M) (Mecp2 Mouse)
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   MBD T158M

   Promoter

   CAGGS

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PMP34-hLOV-TEVcs-GAL4bd-VP64-V5

   Plasmid

   #170997

   Purpose

   HiLITR transcription factor with full-length PMP34 targeting sequence (Peroxisome)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   PMP34(FL)-NNES-hLOV-TEVcs(ENLYFQ-M)-GAL4bd-VP64-V5 (SLC25A17 Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-alpha

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LCV2_mClover3_PSMD1_sgRNA_1

   Plasmid

   #155102

   Purpose

   lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting PSMD1 (core essential gene)

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   PSMD1_sgRNA_1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. LCV2_mClover3_AAVS1_sgRNA_4

   Plasmid

   #155101

   Purpose

   lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLCKO_AAVS1_sgRNA_3

   Plasmid

   #155086

   Purpose

   lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. LCV2_mCherry_AAVS1_sgRNA_4

    Plasmid

    #155106

    Purpose

    lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLCKO_AAVS1_sgRNA_4

    Plasmid

    #155087

    Purpose

    lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. LCV2_AAVS1_sgRNA_4

    Plasmid

    #155090

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV2_hSyn_Aurora_CA_Citrine

    Plasmid

    #98219

    Purpose

    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora_C128A gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

    Promoter

    human synapsin

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_PSG2_WT

    Plasmid

    #81917

    Purpose

    Gateway Donor vector containing PSG2 ,part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PSG2 (PSG2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    V20L; p.333_335del-NPT-ins-IQH

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_SMR3A_WT

    Plasmid

    #81887

    Purpose

    Gateway Donor vector containing SMR3A , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SMR3A (SMR3A Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    C42R; P132L

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_C1orf150_WT

    Plasmid

    #81842

    Purpose

    Gateway Donor vector containing C1ORF150 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    C1ORF150 (GCSAML Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223_SPAG8_WT

    Plasmid

    #81848

    Purpose

    Gateway Donor vector containing SPAG8 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SPAG8 (SPAG8 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    E2D; E5G; P226L

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_CPXCR1_WT

    Plasmid

    #81819

    Purpose

    Gateway Donor vector containing CPXCR1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CPXCR1 (CPXCR1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_OR2M2_WT

    Plasmid

    #81758

    Purpose

    Gateway Donor vector containing OR2M2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    OR2M2 (OR2M2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    R220G, C235R

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223_MTM1_p.R253Q

    Plasmid

    #81724

    Purpose

    Gateway Donor vector containing MTM1, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    MTM1 (MTM1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    R253Q; E410K

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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