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3788 pmko-neo A9-3

Plasmid

#8880

Depositor

Stanley Korsmeyer

Article

Wang et al EMBO J. 2005 Jan 26. 24(2):368-81.

Insert

Hox A9 (HOXA9 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only
pFLAG-4S Tetherin

Plasmid

#41073

Depositor

Paul Spearman

Article

Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

Insert

Tetherin (BST2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S74, S84, 137S, and 144S

Promoter

CMV

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pClneoMyc MOB1 T12A

Plasmid

#47936

Purpose

expresses Myc tagged human MOB1 containing T12A mutation

Depositor

Yutaka Hata

Article

Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

Insert

MOB1A (MOB1A Human)

Tags

Myc

Expression

Mammalian

Mutation

T12A

Promoter

CMV

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
Gr4-T2A-QF2 HDR plasmid

Plasmid

#140944

Purpose

Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Gr4 gene

Depositor

Leslie Vosshall

Article

Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

Insert

Gr4-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr4-right-HDR-arm

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCMX-Gal4-hMeCP2-R111G

Plasmid

#48085

Purpose

Mammalian expression of Gal4 tagged MeCP2-WT-R111G

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Tags

Gal4

Expression

Mammalian

Mutation

R111G mutant MeCP2

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pFLAG-137.144S Tetherin

Plasmid

#41072

Depositor

Paul Spearman

Article

Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

Insert

Tetherin (BST2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

137S, 144S

Promoter

CMV

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pET28-p53 (1-73) (72R)

Plasmid

#62082

Purpose

Expression of human p53 (1-73) (72R) in e. coli

Depositor

Gary Daughdrill

Article

Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

Insert

p53 (TP53 Human)

Tags

HIs

Expression

Bacterial

Mutation

contains TAD residues 1-73; 72R

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
HTRA3_PDZ_1

Plasmid

#103914

Purpose

Protein expression and purification of HTRA3 PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

HTRA3 (HTRA3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40

Plasmid

#180758

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Leu181Lys, Leu…

Promoter

CMV

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET14b-mEKLF

Plasmid

#67834

Purpose

Encodes murine EKLF (nucleotides 71-1215) for in vitro chromatin/transcription reconstitution

Depositor

James Bieker

Article

Armstrong et al Cell. 1998 Oct 2;95(1):93-104.

Insert

EKLF (Klf1 Mouse)

Expression

Bacterial

Mutation

Full-length mEKLF is aa 20-376; amino acid 19 is …

Promoter

T7

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)mGAT1YFP*

Plasmid

#41665

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; C-terminal hyd…

Promoter

CMV

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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miniSOG-Vimentin-7

Plasmid

#57779

Purpose

Localization: Intermediate Filaments, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Vimentin (VIM Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pRD397

Plasmid

#163104

Purpose

Expression of mTurquoise2_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_mTurquoise2_H-NSdbd_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD404

Plasmid

#163109

Purpose

Expression of mNeonGreen_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mNeonGreen_H-NSdbd

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT H335G

Plasmid

#13749

Depositor

Luca Pellegrini

Article

Cipolat et al Cell. 2006 Jul 14. 126(1):163-75.

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

Changed catalytic His 335 to Gly

Available Since

Feb. 1, 2007

Availability

Academic Institutions and Nonprofits only
pCMX-Gal4-hMeCP2-G273X

Plasmid

#48084

Purpose

Mammalian expression of Gal4 tagged MeCP2-G273X

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Tags

Gal4

Expression

Mammalian

Mutation

expresses AT-Hook G273X

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pBa.Halo-KIF13A tail 361-1749

Plasmid

#162720

Purpose

Mammalian expression of WT mouse Kif13A with deletion of the 360 N-terminal residues

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

Kif 13A (Kif13a Mouse)

Expression

Mammalian

Mutation

WT with deletion of the 360 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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ADAP1

Plasmid

#163905

Purpose

Full length mouse ADAP1 for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-vsv-Incenp wt-GFP

Plasmid

#108502

Purpose

inducible expression of vsv-INCENP wt-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFLAG-74.84S Tetherin

Plasmid

#41071

Depositor

Paul Spearman

Article

Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

Insert

Tetherin (BST2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

74S, 84S

Promoter

CMV

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only