We narrowed to 35,234 results for: omp

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HL 1178 (=HL 862 + pHL 282)

Plasmid

#30012

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:mCherry, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
HL 4178 (=HL 862 + pHL 1360)

Plasmid

#30097

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLlacO-1:hfq, pLtetO-1:ompC::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
HL 2314 (=HL 716 + pHL 818)

Plasmid

#30025

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:MicC, pLtetO-1:fhlA::mCherry, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
HL 3612 (=HL 716 + pHL 841)

Plasmid

#30082

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:RhyB-mCherry, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
HL 3619 (=HL 716 + pHL 1228)

Plasmid

#30083

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:DsrA-mCherry, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pSL0527 (pDonor)

Plasmid

#130634

Purpose

Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (Mini-Tn)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ManII-SBP-EGFP

Plasmid

#65258

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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SAP145

Plasmid

#11239

Depositor

Robin Reed

Article

Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

Insert

SAP145 (SF3B2 Human)

Expression

Bacterial

Mutation

First 7 amino acids are missing compared to NP_00…

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

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pET29b-AK1-His

Plasmid

#124135

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Adenylate kinase (AK1 Chicken)

Tags

6x-His

Expression

Bacterial

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ManII-SBP-EGFP

Plasmid

#65252

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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SMT205

Plasmid

#134817

Purpose

SMT201 with mutated RBS for DHFR, in vivo assays

Depositor

Tanja Kortemme

Article

Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

Inserts

Dihydrofolate reductase
Thymidylate synthase

Expression

Bacterial

Mutation

Silent mutations to eliminate internal restrictio…

Promoter

T7 and TET

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ManII-SBP-mCherry

Plasmid

#65253

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pACYC-TtCas6-4xcrRNA4.5

Plasmid

#127764

Purpose

For expression of T. thermophilus Csm complex in E. coli (crRNA)

Depositor

Jennifer Doudna

Article

Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

Inserts

Synthetic CRISPR array containing four identical T. thermophilus spacers
Cas6A

Expression

Bacterial

Mutation

Codon optimized for expression in E. coli

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ManII-SBP-mCherry

Plasmid

#65259

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP) and mCherry

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg7_T

Plasmid

#147556

Purpose

Mammalian Expression of HsSmg7

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7 (SMG7 Human)

Expression

Mammalian

Mutation

two silent mutation G216A, A1521G and one non sil…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSL0915 (CRISPR with target 3)

Plasmid

#130643

Purpose

Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

CRISPR(target-3)

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAdSh.U6.gRNAGFP

Plasmid

#58255

Purpose

U6 promoter-driven single guide RNA complementary to eGFP

Depositor

Manuel Goncalves

Article

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

U6.gRNAGFP cassette

Use

Adenoviral and CRISPR

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pAdSh.U6.gRNAS1

Plasmid

#58252

Purpose

U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

Depositor

Manuel Goncalves

Article

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

U6.gRNAS1 cassette

Use

Adenoviral and CRISPR

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pSL0426 (CRISPR with spc3)

Plasmid

#130642

Purpose

Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

CRISPR(spacer-3)

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsMago_I

Plasmid

#146582

Purpose

Mammalian Expression of HsMago

Depositor

Elisa Izaurralde

Article

Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

Insert

HsMago (MAGOH Human)

Expression

Mammalian

Mutation

one non silent mutation Y123C compared to the seq…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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