We narrowed to 31,166 results for: ica;
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Plasmid#176253PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene and one spacer targeting the LPAAT geneDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-HP1α-miRFP670-Cry2WT
Plasmid#122442PurposeExpresses disordered protein HP1α fused with fluorescent protein miRFP670 and optogenetic protein Cry2WTDepositorInsertHP1 alpha (CBX5 Human)
UseLentiviralTagsCry2WT and miRFP670ExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hlbCas12a-Nlux
Plasmid#176240PurposepNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoterDepositorInserthumanized lbCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterRibosomal subunitAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-gfaABC1D-monSTIM1
Plasmid#161797PurposeLentiviral vector expressing monSTIM1 in astrocytes.DepositorInsertEGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)
UseLentiviralTagsEGFPExpressionMutationPromotergfaABC1DAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
eDrFGFR1
Plasmid#183994PurposeOptically controlled eDrFGFR1 expression in mammalian cellsDepositorInserteDrFGFR1 (FGFR1 Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
eDrHER2
Plasmid#183995PurposeOptically controlled eDrHER2 expression in mammalian cellsDepositorInserteDrHER2 (ERBB2 Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_CCK1.0
Plasmid#208673PurposeExpresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in neuronsDepositorInsertGPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NLS-gs10-cERb447
Plasmid#188257PurposePlasmid ensures constitutive expression of the C-terminal fragment of split ERbeta (aa 447-501), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorInsertcERb447 (ESR1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
ePB-PURO-FLAG-METTL3-APPA
Plasmid#160253PurposeFor stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposaseDepositorInsertmethyltransferase like 3 (METTL3 Human)
UseTagsFLAGExpressionMammalianMutationchanges in aa 395–398, DPPW → APPAPromotertight TRE promoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G_FLAG-CRTC1/MAML2_PGK-GpNLuc
Plasmid#154265PurposeDox/Tet-inducible expression of the CRTC1-MAML2 fusion oncogene in mammalian cells.DepositorInsertFLAG-CRTC1/MAML2 fusion oncogene
UseTagsFLAGExpressionMammalianMutationPromoterTRE3GAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES:mTREK-1(G137I)
Plasmid#133274PurposeMammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tagDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
UseTagsHAExpressionMammalianMutationG137IPromoterCMVd2Available sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer
Plasmid#66779PurposeThe rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signalingDepositorInsertshbeRhGC
red fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET26b(+)-ATI_QconCAT_1
Plasmid#163957PurposeExression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.DepositorInsertATI QconCAT
UseTagspolyhistidine tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_NPYmut
Plasmid#208678PurposeExpresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cellsDepositorInsertGPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_UCN1.0
Plasmid#208668PurposeExpresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in neuronsDepositorInsertGPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1
Plasmid#22008PurposeMammalian expression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
UseRetroviralTagsmVenusExpressionMammalianMutationLOV(Leu404-Leu546), Rac1 starts at I4 and contain…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
LITE2.0_pAAV_hSyn_TALE(Grm2)-GS-CIB1(NLS*,∆318-334)_WPRE_bGHpA
Plasmid#47456PurposeLITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Grm2. Synapsin promoter for neuronal expression.DepositorInsertTALE(Grm2)-GS-CIB1(NLS*,∆318-334)
UseAAV; TaleTagsHAExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-hWIPI1A-3×FLAG
Plasmid#215509PurposeExpresses FLAG tagged human WIPI1A.DepositorInsertWD-repeat protein interacting with phosphoinositides 1A (WIPI1 Human)
UseRetroviralTags3×FLAGExpressionMammalianMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Ma-sfGFP WT
Plasmid#197567PurposeExpression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP WT
M. alvus Pyl-tRNA (6) (4x copies)
UseTagsV5-His6ExpressionMammalianMutationPromoterCMV and U6/H1Available sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CamKIIa-monSTIM1
Plasmid#161796PurposeLentiviral vector expressing monSTIM1 in neurons.DepositorInsertEGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterCamKIIaAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only