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We narrowed to 6,886 results for: GFP expression plasmids

Showing: 1001 - 1050 of 6886 results

  1. p663-UBC-TurboID-V5-eGFP_IDG-K

    Plasmid
    #135264
    Purpose
    Gateway destination clone of eGFP (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-eGFP
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135264-plasmid-map-sequence-id-268064

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  2. p667-UBC-GFP-V5-miniTurbo_IDG-K

    Plasmid
    #135240
    Purpose
    Gateway destination clone of GFP (as control) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    GFP-V5-miniTurbo
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135240-plasmid-map-sequence-id-268088

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  3. hSyn-pDisplay-SNAP[C145A]-T2A-EGFP

    Plasmid
    #223504
    Purpose
    Co-expression of dead-mutant SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker
    Depositor
    James Frank
    Insert
    dead-mutant SNAP-tag and EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SNAP-tag C145A
    Promoter
    hSyn
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    223504-plasmid-map-sequence-id-443304

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  6. pcDNA5/FRT/TO/Flag-tGFP-Cryz

    Plasmid
    #110378
    Purpose
    Mammalian expression of turboGFP-CRYZ with FLAG tag, Flp-In T-Rex system
    Depositor
    Sandra Martha Gomes Dias
    Insert
    crystallin zeta (CRYZ Human)
    Use
    Tags
    FLAG and tGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110378-plasmid-map-sequence-id-213403

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  7. Venus-Bik-L61G-pEGFP-C1

    Plasmid
    #177426
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Leucine 61 replaced with glycine
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177426-plasmid-map-sequence-id-348449

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  8. Venus-Bik-GG153AA-pEGFP-C1

    Plasmid
    #177427
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Glycines 153 and 154 mutated to alanine
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177427-plasmid-map-sequence-id-348452

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  9. Venus-Bik-G154STOP-pEGFP-C1

    Plasmid
    #177428
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Glycine 154 replaced with STOP codon
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177428-plasmid-map-sequence-id-348454

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  25. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

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  26. qTAG-AAVS1-PGK-Puro-moxGFP

    Plasmid
    #227275
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227275-plasmid-map-sequence-id-452039

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  27. AICSDP-116:PCNA-mEGFP

    Plasmid
    #170818
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human PCNA
    Depositor
    Allen Institute for Cell Science
    Insert
    PCNA Homology Arms with mEGFP-linker (PCNA Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    June 11, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    170818-plasmid-map-sequence-id-334731

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  28. pOTTC1584 - pscAAV CMV-IE secreted EGFP-THPKTEL WPRE

    Plasmid
    #188539
    Purpose
    An AAV packaging vector that expresses secreted C-CDNF under control of the EGFP promoter.
    Depositor
    Brandon Harvey
    Insert
    secreted EGFP
    Use
    AAV
    Tags
    CDNF(1-28) and THPKTEL WPRE
    Expression
    Mutation
    Promoter
    CMV-IE
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188539-plasmid-map-sequence-id-370039

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  35. pCRII-TOPO CMV-cGFP-SV40 pA + mMALAT1_3'

    Plasmid
    #91803
    Purpose
    Expresses a cGFP mRNA that ends in the MALAT1 triple helix when the upstream SV40 poly(A) signal fails to be used
    Depositor
    Jeremy Wilusz
    Insert
    Coral Green Fluorescent Protein (cGFP)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    91803-plasmid-map-sequence-id-180321

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  36. pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG

    Plasmid
    #85225
    Purpose
    Expresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassette
    Depositor
    John Naughton
    Inserts
    Optimized G Protein
    Enhanced GFP
    TVA (CD320 Chicken)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Jan. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    85225-plasmid-map-sequence-id-170787

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  37. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64709-plasmid-map-sequence-id-322473

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  38. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid
    #106964
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    106964-plasmid-map-sequence-id-207987

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  39. CMV-H2B-sfGFP-T2A-iCre

    Plasmid
    #183351
    Purpose
    Histone H2B-sfGFP and iCre recombinase
    Depositor
    Mario Capecchi
    Insert
    Histone H2B-sfGFP-T2A-iCre
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    183351-plasmid-map-sequence-id-360158

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  40. integrin beta6 EGFP pWZL Blast2

    Plasmid
    #13591
    Depositor
    Dean Sheppard
    Insert
    integrin beta 6 (ITGB6 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 8, 2007
    Availability
    Academic Institutions and Nonprofits only

  41. PB CAG-sfGFP-GSAx9-iCre-ERT2

    Plasmid
    #137857
    Purpose
    CAG-sfGFP-GSAx9-iCre-ERT2 piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    sfGFP-GSAx9-iCre-ERT2
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    137857-plasmid-map-sequence-id-316181

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  42. XE85 Delta Beta-catenin in GFP

    Plasmid
    #16835
    Depositor
    Randall Moon
    Insert
    Delta beta-catenin GFP (ctnnb1.L Frog)
    Use
    Xenopus expression
    Tags
    GFP
    Expression
    Mutation
    These PCR fragments have the first 138 bp after t…
    Promoter
    Available since
    March 13, 2008
    Availability
    Academic Institutions and Nonprofits only

  43. pETcLIC-GFP

    Plasmid
    #183514
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Use
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    June 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    183514-plasmid-map-sequence-id-362488

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  44. pYUBcLIC-GFP

    Plasmid
    #183509
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Use
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    183509-plasmid-map-sequence-id-362497

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  46. pGG-GFP-PM-TurboID

    Plasmid
    #209402
    Purpose
    Transiently expressing and visualizing the subcellular localization of TurboID fused with a plasma menbrane targeting peptide in planta
    Depositor
    Rosa Lozano-Durán
    Article
    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1
    Insert
    cTP-3XHA-TurboID
    Use
    Tags
    GFP
    Expression
    Plant
    Mutation
    Promoter
    Cauliflower mosaic virus (CaMV) 35S promoter
    Available since
    Jan. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    209402-plasmid-map-sequence-id-418450

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  48. AICSDP-19:LAMP1-mEGFP

    Plasmid
    #101782
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1
    Depositor
    Allen Institute for Cell Science
    Insert
    LAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101782-plasmid-map-sequence-id-198613

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  49. AICSDP-72:TFAM-mEGFP

    Plasmid
    #159745
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TFAM
    Depositor
    Allen Institute for Cell Science
    Insert
    TFAM Homology Arms with linker-mEGFP (TFAM Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Oct. 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    159745-plasmid-map-sequence-id-314375

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  50. MCP-EGFP-PiggyBac

    Plasmid
    #190003
    Purpose
    Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.
    Depositor
    Tineke Lenstra
    Article
    Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi
    Insert
    MCP-EGFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    190003-plasmid-map-sequence-id-375799

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Showing: 1001 - 1050 of 6886 results