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Showing: 401 - 420 of 598 results

  3. pcDNA3.1(+)-FluoSTEP-JNKAR

    Plasmid
    #181857
    Purpose
    Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Article
    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea
    Insert
    FluoSTEP-JNKAR
    Use
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181857-plasmid-map-sequence-id-363201

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  4. pcDNA3.1(+)-FluoSTEP-AktAR

    Plasmid
    #181856
    Purpose
    Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Article
    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea
    Insert
    FluoSTEP-AktAR
    Use
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181856-plasmid-map-sequence-id-362780

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  5. pcDNA3-AMPKsub-YPet-NES

    Plasmid
    #84635
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    84635-plasmid-map-sequence-id-176207

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  7. SERPINA-bio-His

    Plasmid
    #52182
    Purpose
    Expresses full-length Alpha-1-antitrypsin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SERPINA (SERPINA1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52182-plasmid-map-sequence-id-80945

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  13. pcDNA3-ABKAR-Kras

    Plasmid
    #84637
    Purpose
    Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, C…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    84637-plasmid-map-sequence-id-176126

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  14. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid
    #138208
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKCsub(T/A)-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    138208-plasmid-map-sequence-id-307411

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  15. pcDNA3-AMPKsub-YPet-Kras

    Plasmid
    #84638
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub-YPet-Kras
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    84638-plasmid-map-sequence-id-176882

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  16. GB4635

    Plasmid
    #215263
    Purpose
    Module for the expression of HISPS under the copper inducible CBS:mDFR promoter and H3H under the red light regulated Etr8:CMVmin promoter and the constitutive expression of LUZ, CPH,EGFP and p19
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    CBS4x:mDFR:HISPS-Etr4:mDFR:H3HSF-LUZ-CPH-EGFP-p19
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    215263-plasmid-map-sequence-id-460239

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  19. GB4636

    Plasmid
    #215264
    Purpose
    Module for the expression of HISPS under the ed light regulated Etr8:CMVmin promoter and H3H under the copper inducible CBS:mDFR promoter and the constitutive expression of LUZ, CPH,EGFP and p19
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    Etr4:mDFR:HISPS-CBS4x:mDFR:H3HSF-LUZ-CPH-EGFP-p19
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    215264-plasmid-map-sequence-id-440689

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Showing: 401 - 420 of 598 results