We narrowed to 2,477 results for: LARGE

1. cr3Dd

   Plasmid

   #184862

   Purpose

   cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   Cas9, bet, exo
   

   Use

   Tags

   Cas9 ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWS5397-CRISPRai-NatR

   Plasmid

   #182836

   Purpose

   Saccharomyces cerevisiae inducible CRISPRai vector - NatR marker

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH6HTC_ENO1-Halo

   Plasmid

   #175330

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   ENO1 (ENO1 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CAV1-mCherry

   Plasmid

   #27705

   Depositor

   Ari Helenius

   Article

   Hayer et al Traffic. 2010 Mar . 11(3):361-82.

   Insert

   caveolin-1 (CAV1 Canis lupus familiaris)
   

   Use

   Tags

   monomeric Cherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pH6HTC_CCT2-Halo

   Plasmid

   #175337

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   CCT2 (CCT2 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pH6HTC_CCT3-Halo

   Plasmid

   #175322

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   CCT3 (CCT3 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJEP207-AAV-CMV-MCS-pA

   Plasmid

   #78318

   Purpose

   AAV vector backbone containing a CMV promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Cytomegalo Virus(CMV)

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

   Plasmid

   #55222

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLSSmOrange-mKate2 caspase-3 biosensor

   Plasmid

   #37132

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

   Insert

   LSSmOrange-DEVD-mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

   Promoter

   CMV

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTrichoGate-5

    Plasmid

    #160705

    Purpose

    Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    Trichoderma optimised Synthetic eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFnCpfAb-apr

    Plasmid

    #214198

    Purpose

    Expresses FnCpf1 and RecAb recombination system for genome editing in Acinetobacter baumannii

    Depositor

    Yu Wang

    Article

    Wang et al Curr Issues Mol Biol. 2024 Jan 5;46(1):5

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    tac promoter

    Available since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAV1-P132L-mEGFP

    Plasmid

    #27708

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    caveolin-1 (CAV1 Canis lupus familiaris)
    

    Use

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    P132L

    Promoter

    Available since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac His6 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-B)

    Plasmid

    #55219

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWS3799-gRNA-Csy4-template

    Plasmid

    #182827

    Purpose

    SpCas9 gRNA-Csy4 template

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Insert

    SpCas9 gRNA scaffold + Csy4 sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLOX-Ttag-iresTK

    Plasmid

    #12246

    Depositor

    Didier Trono

    Article

    Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

    Insert

    SV40 Large T, HSV1-TK (SV40gp6 Simian virus)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    LoxP in 3' LTR.

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH6HTC_BRD2-Halo

    Plasmid

    #175336

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    BRD2 (BRD2 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWS5399-CRISPRai-ZeoR

    Plasmid

    #182838

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pH2B-LSSmKate2-N1

    Plasmid

    #31903

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    H2B-LSSmKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

    Promoter

    CMV

    Available since

    Sept. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. p5155

    Plasmid

    #175391

    Purpose

    Low Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)

    Depositor

    Michael Wiles

    Article

    Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

    Type

    Empty backbone

    Use

    Mouse Targeting; Cloning vector, to be used to de…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJEP217-Lenti-CMV-GFP-WPRE-pA

    Plasmid

    #62925

    Purpose

    pLenti vector backbone designed to express GFP from a CMV promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    1.3αCaMKII and CMV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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